安络化纤丸对二甲基亚硝胺诱导大鼠肝纤维化的抑制作用

Inhibitory effect of anluohuaxianwan on experimental hepatic fibrosis in rats

目的 观察安络化纤丸对二甲基亚硝胺诱导大鼠肝纤维化形成的抑制作用。方法采用二甲基亚硝胺诱导大鼠肝纤维化模型，观察安络化纤丸干预后大鼠肝功能、血清透明质酸（HA）和肝组织羟脯胺酸含量变化，并观察肝组织病理学改变和基质金属蛋白酶2（MMP2）的表达情况。多组计量资料分析采用OnewayANOVA，多重比较采用LSD方法。结果安络化纤丸干预组血清ALT、AST水平分别为（129．08±53．45）U／L和（321．25±138．32）U／L，血清HA和肝组织羟脯胺酸含量分别为（1644．47±380．45）ng／ml和（0．23±0．08）μg／mg，均明显低于模型组【分别为（611．77±354．17）U／L，（1199．00±763．54）U／L，（3768．38±851．98）ng／ml，（0．51±0．13）ug／mg】，差异均具有统计学意义（P〈0．01）。光镜下显示安络化纤丸干预组大鼠肝组织病理改变较模型组明显减轻（P〈0．01），肝组织MMP2表达强度明显高于模型组（P〈0．05）。结论安络化纤丸对二甲基亚硝胺诱导的大鼠肝纤维化形成有较好的抑制作用，其可能的机制与保肝降酶、增强肝组织MMP2活性和促进细胞外基质降解有关。

Objective To investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine （DMN） in rats. Methods 36 male SD rats were randomly dividied into three groups： model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 （MMP-2） in liver tissue were detected by immunohistochemistry. Results The serum levels ofALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group （P 〈 0.01）. The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group （P 〈 0.01）. The liver MMP-2 in the model group was significantly increased compared to that in the normal group （P 〈 0.05）. The expression of MMP-2 in liver tissue of model group was lower than that in the anluohuaqianwan group （P 〈 0.05）. Conclusion Anluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.