两种细胞制作的人肝癌裸鼠模型转移性状的比较

Comparison of the metastatic characteristics of HCCLM3 cells and SMCC-7721 cells in nude mice model

目的 比较两种人肝癌细胞株（HCCLM3和SMMC-7721细胞）构建的人肝癌裸鼠模型的转移潜能。方法实验分为两组：应用HCCLM3细胞构建的人肝癌裸鼠模型组为A组，SMMC7721细胞构建的模型组为B组。比较A组和B组的原发灶的肿瘤大小和病理检查结果。比较各组的肝内转移率、转移数目，累及的肝叶数以及转移肿瘤体积的大小。所有数据由SPSS16．0软件分析，计量资料采用t检验，率的比较采用Fisher确切概率法，计数资料采用秩和检验。结果A组的肝内转移率为100％（8／8）、肝内原发肿瘤体积为（6954±1945）mm^3，B组的肝内转移率为62．5％（5／8）、肝内原发肿瘤体积为（6034±2035）mm^3，两组肝内转移率和原发灶肿瘤体积大小比较，差异无统计学意义。肝内转移的数目、累及肝叶数、转移肿瘤的体积大小A组分别为4．5个（中位数）、3个和975mm^3（中位数），B组分别为1个（中位数）、1个和274mm^3（中位数），两组比较，Z值分别为-2．818、2．289和1．975，P值均〈0．05，差异均有统计学意义。两组在肺转移率（P=0．001）和累及其他脏器个数（P=0．041）的比较，差异均具有统计学意义。结论HCCLM3细胞制作的人肝癌裸鼠转移模型具有高转移潜能，可应用于人肝癌的生物性状的研究。

Objective To compare the metastatic characteristics of HCCLM3 cells and SMCC-7721 cells in nude mice model. Methods Nude mice were divided into two groups （n = 8, each）, mice were transplanted with HCCLM3 ceils （group A） and SMMC-7721 cells （group B）. Tumor size, metastasis rate and other clinical parameters were compared between the two groups. Statistical analysis Was performed with the help of SPSS 16.0 for Windows computer software （SPSS, Inc., Chicago, IL）. P values of less than 0.05 were considered statistically significant. Results Intrahepatic metastases rate was 100% （8 / 8）, mean intrahepatic primary tumor volume was （6954 ± 1945） mm^3 in group A, Intrahepatic metastases rate was 62.5% （5/8）, and mean intrahepatic primary tumor volume was （6034±2035） mm3 in the group B. There was no statistical difference in the primary liver tumor size and intrahepatic metastases rate （P = 0.20; t = 6.38, P = 0.37, respectively）. The numbers of intrahepatic metastases and the involved lobes, and the volume of tumor were 4.5 （median）, 3, and 975 mm3 （median） respectively, in group A, and these were 1 （median）, 1 and 274 mm3 （median） respectively in group B. The difference between two groups was statistically significant （Z values, -2.818, -2.289, and -1.975, repectively）.The rate of lung metastasis and other organ metastasis in the A group was significantly higher than that in group B （P 〈 0.001, P 〈 0.041, respectively）. Conclusion HCCLM3 cells have higher metastatic potential than SMMC-7721 cells in nude mice.