32株麻风菌rpoT基因和SNP基因分型

Genotyping of rpoT gene and SNP on M.leprae isolates in China

目的:了解中国麻风菌株rpoT和SNP基因型特征及地理分布。方法:从32例中国麻风病患者皮损组织中提取DNA。rpoT基因分型采用PCR-直接测序;SNP分型采用PCR-直接测序和PCR-限制性片段长度多态性分析。结果:32份中国菌株的SNP均为3型。32份菌株中,来自我国东南部和藏族地区的12份菌株rpoT基因为4个拷贝;来自云贵川的20份菌株rpoT基因为3个拷贝。结论:32份菌株均为SNP3型,但我国不同地区rpoT基因型不一致,有待进一步研究。

Objective： To understand the characteristics and geographical distribution of the genotypic profiles of M. leprae based on rpoT and SNP in China. Methods： M. leprae DNA was extracted from skin biopsies of thirty - two leprosy patients rpoT genotyping was performed by PCR- sequencing and SNP genotyping by PCR- sequencing and PCR - RFLP. Results： All the thirty - two isolates were SNP type 3 but the allele for rpoT gene was three or four - copy. Of the thirty - two isolates, twelve f？om the southeast region and Tibet were four- copy; twenty isolates from Yunnan, Guizhou and Sichuan three- copy in rpoT gene. Conclusion： The 32 isolates were SNP type 3 but the rpoT allele of M. leprae obtained from different regions is different. The reason is not clear and the distribution of rpoT allele needs to be studied in a large sample.