完全与不完全睡眠剥夺对小鼠免疫功能的影响

Effects of complete and incomplete sleep deprivation on immune function of mice

目的:研究经完全与不完全睡眠剥夺以后小鼠免疫功能的变化。方法:通过光照模拟全白天生活、黑箱模拟全黑夜生活,连续2周干扰小鼠睡眠;通过转轮24 h和72 h短期全剥夺小鼠睡眠;实验小鼠实施睡眠干扰前以BSA免疫一次。采用称重脾脏;流式细胞术(FCM)检测小鼠外周血CD3+、CD4+、CD8+T淋巴细胞数量及其比例;以ELISA方法检测血清中IL-2、IL-10以及抗BSA特异性抗体含量。结果:与正常睡眠小鼠相比:全日组脾重没有显著变化,其余各组脾重明显减轻(P<0.05);24 h转轮组CD4+T细胞数明显降低(P<0.05),其他各组没有明显变化(P>0.05);所有实验组CD8+T细胞数均明显降低,CD4/CD8比例显著增大(P<0.01);所有实验组IL-2下降(P<0.01);除全日组IL-10含量升高以外(P>0.05),其他实验组IL-10含量均呈下降趋势(P<0.05);转轮组小鼠抗BSA抗体效价含量显著降低(P<0.01),全日光组与全夜组无统计学意义。结论:完全与不完全睡眠剥夺可不同程度地引起实验小鼠CD8+T细胞数减少、脾重减轻、特异性抗体分泌减少以及细胞因子分泌格局的变化,进而影响机体免疫功能。

AIM：To study the interaction between sleep deprivation and immune functions in mice.METHODS：To set up the uncompleted sleep deprivation of mice by means of lighting mimicked with whole day and dark box done with whole night for 2 weeks and to deprive the mice of sleep in the rotating cage for 24 h and 72 h.All mice are challenge with BSA before sleep deprivation.The spleen weight of mice was measured by analytic balance.The count of T subpopulation was detected with FACS.The levels of cytokines IL-2,IL-10 and the concentration of the specific antibody to BSA was detected by ELISA method.RESULTS：Compared with the normal control,the spleen weight of mice in all the other experimental groups was decreased（P〈0.05）except those of lighting group.Decrease of CD8＋ T lymphocyte was observed（P〈0.01） while the ratio CD4/CD8 increased greatly.Augmentation of IL-10 was demonstrated only in the all-light group,both IL-2 and IL-10 decreased（P〈0.01） in the other groups.Significant decrease of specific antibody for BSA was found in the mice of sleep deprived group（P〈0.01）.CONCLUSION：Complete and incomplete sleep deprivation inhibit differently the immune function of mice by triggering decrease of CD8＋ Tcells,inhibiting secretion of specific antibody and causing disturbance on the pattern of cytokines.