PCNA和Caspase-3在肺癌组织中的表达及意义

Expression and significance of PCNA and Caspase-3 in the tissue of lung cancer

目的:探讨增殖细胞核抗原(PCNA)和天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)在肺癌中的表达及其临床意义。方法:应用免疫组化SP法对80例肺癌病例和相对应的80例正常肺组织进行PCNA蛋白和Caspase-3蛋白半定量分析。结果:PCNA蛋白在肺癌组织中阳性表达率为65.0%,Caspase-3在肺癌组织中阳性表达率为32.5%,与相应正常肺组织中的阳性表达率有显著差异(P<0.05)。PCNA蛋白的表达与淋巴结转移、分期和分化程度明显相关(P<0.05),Caspase-3蛋白表达与肺癌的组织病理学类型、淋巴结转移以及分化程度明显相关(P<0.05),与病理分期却未表现出相关性(P>0.05),PCNA蛋白与Caspase-3蛋白的表达呈负相关,这其中PCNA(+)Caspase-3(-)的肺癌群体低/未分化危险性是非PCNA(+)Caspase-3(-)的27倍(OR=27.0,P<0.05)。结论:肺癌组织PCNA蛋白表达增强和Caspase-3蛋白表达减弱,两者的表达与肺癌的病理类型,淋巴结转移,分期和分化程度具有一定关系,关于这两种蛋白的研究对临床工作有一定指导意义。

AIM：To investigate the significance of PCNA and Caspase-3 in lung cancer.METHODS：PCNA and Caspase-3 in 80 patients diagnosed with lung cancer were semi-quantitatively analyzed by using SP immunohistochemical staining.RESULTS：The positive rate of Caspase-3 and PCNA were 32.5% and 65%,respectively.Both were much lower or higher than those normal lung tissues（P〈0.05）.Caspase-3 protein was correlated to lymph node metastasis,pathological type and differentiation grade（P〈0.05）.Meanwhile,PCNA was correlated to lymph node metastasis,differentiation grade and pathological staging of lung cancer（P〈0.05）,but not to pathological type.PCNA was negatively correlated to Caspase-3.PCNA（＋）/Caspase-3（-） indicated the potential lower differentiation grade.CONCLUSION：Up-regulation of PCNA and down-regulation of Caspase-3 are correlated to the pathological type,lymph node metastasis,staging and differentiation grade in lung cancer,especially which is indicating its clinical treatment.