C57BL/6小鼠皮肤毛囊发育的实验研究

The experimental study of C57BL/6 mouse skin hair follicle development

目的探讨胎鼠,乳鼠和成鼠毛囊发育的规律。方法石蜡和冰冻超薄切片,采用HE染色,AP活性染色,油红O染色,凋亡细胞鉴定等方法观察不同时期C57BL/6小鼠毛囊的发育情况。结果胚胎发育第15.5d(E15.5)开始出现毛囊,至E18.5胎鼠背部皮肤逐渐增厚,毛囊逐渐增多。乳鼠出生第1d毛囊大部分处于第4阶段之前,毛囊数量继续增加,至出生第4d皮肤基底层未见新生的毛囊,此时毛囊数量维持不变,出生第9d毛囊达到第8阶段。成鼠脱毛后第9d约有95%的毛囊进入生长Ⅳ期,第17d约有63.3%的毛囊进入退化期,第22d约有85%的毛囊处于退化期。结论小鼠毛囊的早期发育起始于E15.5,从E17.5到出生后3d毛囊数量呈快速增加趋势,因此,这一时期可以用于探讨毛囊再生机制等相关研究。

Objective To investigate hair follicle pattern of fetal,neonatal and adult mouses.Methods The different development process of hair follicle in C57BL/6 mice were observed using several methods,including paraffin and frozen ultrathin sections,HE staining,AP activity staining,oil red O staining and apoptotic cells identification.Results The hair follicles section started to develop in the E15.5 fetal mouse of C57BL/6 and the back skin became thicker gradually in the E18.5 fetal mouse with more hair follicles appeared.The most of hair follicles of the 1st day suckling mice born would grow into the fourth stage of hair follicle development with the numbers of hair follicles increasing quickly.At the 4th day after born of immature mice,the most of hair follicles continued to develop,but the number of hair follicles remained unchange.The hair follicles of the 9th day suckling mice would grow into the 8th stage of hair follicle development.For 9d adult mouse after depilation,95% of the hair follicles entered the growth stage Ⅳ,in the first 17d,63.3% of the hair follicles entered the degradation period and in the first 22d of,85% of the hair follicles grew into the degradation period.Conclusions The hair follicle of fetal mouse starts at E15.5 and from E17.5 to 3 days after birth.The number of hair follicles will increase rapidly,therefore,this period can be used to explore the mechanism of hair follicle regeneration.