人脐血单个核细胞和脐带间充质干细胞移植用于大鼠脊髓损伤的实验研究

The study of human umbilical cord blood mononuclear cells and human umbilical cord mesenchymal stem cells transplantation in the treatment of spinal cord injury

目的:探讨人脐血单个核细胞和脐带间充质干细胞(MSCs)移植对脊髓损伤功能恢复的影响,寻找一种更适合治疗脊髓损伤的细胞源。方法:采集新鲜人脐带血和脐带,分离培养单个核细胞和MSCs。将脊髓损伤模型随机分成3组:单个核细胞移植组、MSCs移植组和低糖必需培养基(L-DMEM)培养组。采用免疫组化和免疫荧光检测细胞移植后1—4周细胞在脊髓内的存活情况和迁移情况,使用BBB行为学评分评估大鼠脊髓功能恢复情况。结果:L-DMEM培养液组在术后各时间点观察评分无明显差异,而细胞移植组脊髓功能处于逐渐恢复过程,与L-DMEM培养液比较,差异有显著性意义。单个核细胞移植组对损伤脊髓功能的修复作用较MSCs移植组显著,且差异有显著性意义。结论:与MSCs相比较,人脐血单个核细胞更适合作为治疗脊髓损伤的细胞源。

Objective：To explore the effect of human umbilical cord mononuclear cells and human umbilical cord mesenchymal stem cells （MSCs） transplantation on injured spinal cord function and to find a better source of cells for the treatment of spinal cord injury（SCI）.Method：Human umbilical cord blood and human umbilical cords were collected,and mononuclear cells and MSCs were derived.The rat SCI models were divided into 3 groups：SCI model plus mononuclear cells transplantation group,SCI model plus mesenchymal stem cells transplantation group and SCI model plus L-DMEM group.Immunohistochemistry and immunofluorescence were used to determine the survival and migration of implanted cells in spinal cord 1—4 weeks after transplantation.Basso-Beattie-Brasnahan （BBB） scale system was applied to assess the function recovery of spinal cord.Result：There was no significant difference in postoperative score at each time point in SCI model plus DMEM group.The function of spinal cord became better in SCI model plus cells transplantation groups,and there were significant differences compared with SCI model plus DMEM group.The function of spinal cord in SCI model plus mononuclear cells transplantation group was better than that in SCI model plus MSC transplantation group,and the difference was prominently.Conclusion：Compared with MSCs,human umbilical cord blood mononuclear cells were more suitable as a source of cells for the treatment of SCI.