胰星状细胞对胰腺癌细胞株Patu8988侵袭和转移的影响

Pancreatic stellate cells promote the invasion and migration of human pancreatic cancer Patu8988 cells

目的:探讨永生化人胰星状细胞(IPSC)对胰腺癌细胞株Patu8988侵袭和转移的影响.方法:制备IPSC上清,用IPSC上清和Patu8988共培养,以单独培养的Patu8988为对照,比较实验组与对照组细胞的增殖、黏附能力,侵袭、迁移能力,克隆形成以及抵抗H2O2诱导的Patu8988凋亡能力.结果:与单独培养的胰腺癌细胞株Patu8988相比,IPSC上清对胰腺癌细胞株Patu8988细胞的增殖、黏附、迁移、侵袭能力及克隆形成能力有促进作用(均P<0.05),并能抑制H2O2诱导的Patu8988的凋亡(P<0.05).结论:胰星状细胞可能通过增强胰腺癌细胞株Patu8988的增殖、黏附、迁移、侵袭能力及克隆形成能力,并抑制其的凋亡,在胰腺癌的发展和转移中起重要作用.

AIM：To investigate the effects of immortalized pancreatic stellate cells（IPSCs） on the invasion and migration of human pancreatic cancer Patu8988 cells.METHODS：After Patu8988 cells were incubated with the culture supernatant of IPSCs, their growth, adherence, invasion, migration, clonogenicity and resistance to hydrogen peroxide（H2O2 ）-induced apoptosis were measured.RESULTS：Compared with untreated Patu8988 cells, cell growth, adherence, invasion, migration and clonogenicity were promoted（all P 0.05）, and H2O2-induced apoptosis（P 0.05） was inhibited in Patu8988 cells incubated with the supernatant of IPSCs.CONCLUSION：Pancreatic stellate cells may exert an important role in the progression and metastasis of pancreatic cancer by promoting the growth, adherence, invasion, migration and clonogenicity of Patu8988 cells and inhibiting their apoptosis.