长期应用川芎嗪对大鼠结肠黏膜阴离子分泌的影响

Long-term use of ligustrazine suppresses anion secretion in rat colonic mucosa

目的:探讨长期应用川芎嗪对大鼠结肠黏膜阴离子分泌的影响.方法:健康SD大鼠分为两组:川芎嗪组[腹腔注射川芎嗪40mg/(kg·d)]和对照组(腹腔注射等量生理盐水),7d后剥离结肠黏膜,采用短路电流技术并运用吲哚美辛和forsklin观察大鼠结肠黏膜阴离子的分泌.结果:对照组和川芎嗪组结肠黏膜的基础电流相比有显著差异(23.4μA/cm2±2.2μA/cm2vs18.1μA/cm2±2.2μA/cm2,P<0.05);预先加入前列腺素环化酶抑制剂-吲哚美辛后,对照组和川芎嗪组结肠黏膜的基础电流相比无显著差异;对照组和川芎嗪组分别加入腺苷酸环化酶的激动剂-forskolin后,30min内转运的电荷数的增加量有显著差异(47.9mC/cm2±3.6mC/cm2vs27.1mC/cm2±2.6mC/cm2,P<0.01);而川芎嗪组结肠黏膜30min内转运的电荷数与川芎嗪组单纯加入forskolin后的短路电流相比差异无显著性.结论:川芎嗪可通过抑制结肠黏膜对前列腺素的自发分泌来降低阴离子的分泌.

AIM：To investigate the effects of long-term use of ligustrazine on anion secretion in rat colonic mucosa.METHODS：Healthy Sprague-Dawley rats were divided into two groups：ligustrazine treatment group and control group.The treatment group and control group were injected intraperitoneally with ligustrazine 40 mg/（kg？d） and normal saline 2 mL/（kg·d）, respectively.After treatment for 7 consecutive days, the anion secretion in colonic mucosa was investigated using shortcircuit current（Isc） technique.RESULTS：The basal current（BC） in colonic mucosa in the ligustrazine treatment group was significantly lower than that in the control group（23.4 μA/cm2 ± 2.2 μA/cm2 vs 18.1 μA/cm2 ± 2.2 μA/cm2 , P 0.05）.However, no significant dif-ference was noted in the BC in colonic mucosa pretreated with indomethacin（10 μmoL/L）, a prostaglandin synthesis inhibitor, between the two groups.After addition of forskolin, an activator of adenylyl cyclase, the increase in charge density in 30 minutes was higher in the control group than in the ligustrazine treatment group（47.9mC/cm2 ± 3.6m C/cm2 vs 27.1 mC/cm2 ± 2.6m C/cm2,P 0.01）.In contrast, no significant difference was noted in forskolininduced increase in charge density in colonic mucosa pretreated with indomethacin between the two groups.CONCLUSION：Long-term treatment with ligustrazine inhibits anion secretion in rat colonic mucosa by decreasing the synthesis of prostaglandin.