人T细胞识别的鳞状细胞癌抗原3基因疫苗肿瘤免疫研究

Tumor immunological study of gene vaccine of human SART3

目的观察人T细胞识别的鳞状细胞癌抗原3(SART3)基因的DNA疫苗能否诱导小鼠产生针对表达该基因肿瘤细胞的免疫反应。方法构建表达人SART3基因的C3H小鼠肿瘤细胞LM8-SART3。以空载体为对照,注射疫苗,分离小鼠脾细胞,体外检测CTL反应。于疫苗免疫后2周接种肿瘤细胞,比较免疫组与空载体组肿瘤长出及出瘤后肿瘤生长情况。结果体外可检测到疫苗诱导的小鼠脾细胞CTL活性;疫苗注射后小鼠成瘤率降低,肿瘤生长慢于对照组。结论表达人SART3基因的DNA疫苗可在一定程度上预防表达该基因的肿瘤细胞LM8-SART3在小鼠体内的成瘤及生长。

Objective To study whether the gene vaccine of human SART3 （the squamous-cell carcinoma antigen recognized by T cells-3） can induce the mouse to show immunological response to the tumor cells. Methods Mouse tumor cells expressing human SART3 gene （LM8-SART3） were constructed. The C3H mice were immunized with gene vaccine, from which splenocytes were isolated and cytotoxic T lymphocytes （CTL） activity was measured, with the same amount vacant plasmid pEFG-N1 as control. The immunized C3H mice were also challenged by transplanting LM8-SART3 cells; the tumor occurrence and tumor growth in immunity group and control was recorded. Results Gene vaccine expressing human SART3 elicited CTL reaction of mouse splenocytes, after vaccination, in vivo tumor occurrence and tumor growth speed decreased. Conclusions Gene vaccine expressing human SART3 elicited specific immunity and suppressed tumor cells expressing human SART3 （LM8-SART3） in vitro and in vivo, which may provide a new way in tumor prevention and cure.