狂犬病毒分离株糖蛋白基因的克隆与序列分析

Cloning and analysis of glycoprotein gene of rabies virus isolated from Guangdong province

为分析狂犬病毒(RV)分离株糖蛋白基因之间的差异,本研究采用RT-PCR的方法扩增由广东某地临床表现正常犬的唾液中分离的RV(GD33)分离株的G基因,将其克隆、测序后与国内外部分毒株相应基因进行核苷酸序列比较,结果表明,该分离株与其他毒株相比核苷酸同源性为94%～97.1%;氨基酸同源性为97%～99.2%。而GD33分离株与HEP-Flury株在跨膜区、膜内区有很高的一致性;另外,GD33分离株与HEP-Flury和FluryLEP株在333位点出现精氨酸被取代的现象,证实GD33分离株这2个疫苗株没有显著差异,对监控我国狂犬病疫情具有一定的意义。

The glycoprotein （G） gene of rabies virus （GD33）, isolated from salivary of a health dog in Guangdong province, was amplified by RT-PCR, cloned and sequenced. Comparison results showed that the nucleotides and deduced amino acids sequence of G genes of GD33 and HEP-Flury share 97.1% and 99.2 % homology, respectively, especially in transmembrane and intramembrane regions. In addition, an Arg to Gln mutation was found in G gene of GD33, HEP-Flury and FluryLEP strains at the 333 amino acid site. These results indicated that the GD33 isolate is low virulent strain.