大鼠睾丸组织COX Ⅶ a表达的加龄变化及与睾酮合成的相关性

Studies on the Age-related Changes on COX Ⅶ a mRNA and Protein and the Correlation between COX Ⅶ a and Testosterone Production in Rat Testicular Tissue

目的:探讨大鼠睾丸组织COXⅦa(cytochrome C oxidase Ⅶ a)表达的加龄变化及其与睾酮合成之间的相关性。方法:①选用出生后1周、3周、5周、3月和24月龄雄性SD大鼠,每组6只。②采用半定量RT-PCR法检测大鼠睾丸组织COXⅦamRNA的表达。③Western blotting方法检测大鼠睾丸组织COXⅦa蛋白表达。④化学发光法检测大鼠血清睾酮浓度。结果:①血清睾酮浓度3月、24月、5周、3周和1周龄组依次降低,其中3月龄和24月龄组与其余各组间差异有统计学意义(P<0.05)。②COXⅦamRNA水平24月、1周、3周、5周和3月龄组依次降低,其中3月龄和24月龄组与其余各组之间差异有统计学意义(P<0.05)。③睾丸组织COXⅦa蛋白表达,24月龄组最强,3月龄组最弱,除1周龄和3周龄组之间差异无统计学意义外,其余各组之间差异均有统计学意义(P<0.05)。④睾酮浓度与COXⅦamRNA水平和蛋白表达呈负相关(r=-0.580,P=0.013;r=-0.447,P=0.000),而COXⅦamRNA水平和蛋白表达呈正相关(r=0.941,P=0.000)。结论:COXⅦa随增龄变化,老年大鼠COXⅦa显著上调,与睾酮合成能力下降有关,可能成为治疗PADAM的候选基因。

Objective： To investigate the effects of age-related changes on COX VII a mRNA and Protein in rat testicular tissue and the correlation between COX VII a and testosterone prodution. Methods： Male SD rats aged 1 week, 3 weeks, 5 weeks, 3 months and 24 months were studied. The serum testosterone was determined by chemiluminescence immunoassay （CLIA）, COX VII a mRNA and protein was determined by semiquantitative RTPCR and Western blotting respectively. Results： 1） The level of testosterone of 3 months （the highest） and that of 24 months （the second） among groups were great significant compared with that of other groups respectively （P〈0.05）. 2） The level of COX VII a mRNA of 24 months group was significantly higher and that of 3 months was significantly lower compared with other groups respectively （P〈0.05）. 3） The COX VII a protein expression was corresponded with the level of COX VII a mRNA except that of 5 weeks group was significantly lower compared with those of 1 week and 3 weeks groups （P〈0.05）. 4） The level of testosterone was negatively correlated with COX VII a mRNA and protein （r =-0.580, P=0.013; r =-0.447, P=0.000）, and the level of COX VII a mRNA was positively correlated with the expression of COX VII a protein （r =0.941, P=0.000）. Conclusion： The expression of COX VII a in aged male testis was changed significantly compared with those in young groups, implying that COX VII a may be a candidate gene for treatment of PADAM.