酒精性肝病患者淋巴细胞染色体损伤与氧化应激的关系

Relationship between chromosome damage in lymphocytes and oxidative stress in patients with alcoholic liver disease

目的:探讨酒精性肝病(alcoholic liver disease,ALD)患者外周血淋巴细胞染色体损伤与体内氧化应激之间的关系.方法:采用胞质分裂阻滞法微核实验、硫代巴比妥酸显色法和黄嘌呤氧化酶法分别检测31例ALD患者和22例健康体检者外周血淋巴细胞染色体损伤情况、血浆丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性.结果:病例组与对照组血浆MDA含量分别为5.88μmol/L±2.62μmol/L和2.89μmol/L±1.33μmol/L,SOD活性分别为49.28kU/L±6.03kU/L和57.35kU/L±4.96kU/L,血浆MDA含量随着SOD活性的降低而增高,两组各指标之间有显著性差异(P<0.01),且病例组MDA含量与SOD活性变化之间呈负相关(r=-0.454,P<0.05).此外,病例组微核率高于对照组,两组相比有显著性差异(P<0.01).病例组MDA含量与微核率存在正相关(r=0.493,P<0.01),而SOD活性与微核率存在负相关(r=-0.422,P<0.05).结论:ALD患者体内氧化应激在外周血淋巴细胞染色体损伤中起重要作用,提示ALD患者体内的氧化应激是其外周血淋巴细胞染色体损伤的机制之一.

AIM To explore the relationship between chromosome damage in peripheral blood lymphocytes and oxidative stress in patients with alcoholic liver disease （ALD）. METHODS： Thirty-one ALD patients （experimental group） and 22 healthy volunteers （control group） were included in the study. Chromosome damage in peripheral blood lymphocytes was detected by cytokinesis-block micronucleus test. Plasma malondialdehyde （MDA） content was determined by the thiobarbituric acid （TBA） method, and superoxide dismutase （SOD） activity was measured by the xanthine oxidase method. RESULTS： The content of plasma MDA was significantly higher in the experimental group than in the control group （5.88 μmol/L ± 2.62 μmol/L vs 2.89 μmol/L ± 1.33 μmol/L, P 〈 0.01）, while the activity of SOD was significantly lower in the experimental group than in the control group （49.28 kU/L ± 6.03 kU/L vs 57.35 kU/L ± 4.96 kU/L, P 〈 0.01）. A negative correlation was noted between the content of plasma MDA and SOD activity （r = -0.454, P 〈 0.05） in both groups. The rate of micronucleus formation was significantly higher in the experimental group than in the control group （P 〈 0.01）. A positive correla- tion between the content of MDA and the rate of micronucleus formation （r = 0.493, P 〈 0.01）, and a negative correlation between SOD activity and the rate of micronucleus formation were found in the experimental group （r = -0.422, P 〈 0.05）. CONCLUSION： Oxidative stress plays an important role in chromosome damage in peripheral blood lymphocytes in patients with alcoholic liver disease, suggesting that oxidative stress is one of the mechanisms causing chromosome damage in peripheral blood lymphocytes in these patients.