宫颈阴道组织水通道蛋白-2的表达水平及其相关因素研究

The mRNA Expression and Correlate Factors of AQP2 in Human Ectocervical-vaginal Epithelial Cell

目的:探讨人宫颈阴道上皮组织中水通道蛋白-2(AQP2)mRNA的表达。方法:采用实时荧光定量PCR技术检测2007年3月至2008年3月首都医科大学附属复兴医院妇产科45例患者(月经正常女性18例,围绝经期女性14例,绝经后期女性13例)宫颈阴道组织AQP2mRNA的表达水平,应用单因素方差分析及一元线性相关与回归进行分析。结果:AQP2mRNA在宫颈阴道上皮中均有表达,月经正常组、围绝经组及绝经组宫颈阴道组织中AQP2mRNA表达量依次降低,月经正常组AQP2mRNA表达水平强于围绝经组和绝经组,差异有统计学意义(P<0.05),围绝经组和绝经组比较,差异无统计学意义(P>0.05),同时研究发现AQP2mRNA表达与年龄呈负相关(r=-0.479,P<0.05),与血浆渗透压呈负相关(r=-0.285,P<0.05),与E2水平呈正相关(r=0.530,P<0.05)。结论:围绝经期及绝经后期宫颈阴道上皮中AQP2mRNA的表达下降,可能导致阴道分泌物减少,是引起围绝经期及绝经后期阴道干涩等症的可能机制之一。

Objective： mRNA expression of aquaporin-2 （AQP2） in human ectocervical-vaginal epithelial cell （hECE） was investigated. Methods： 45 women were recruit from Capital Medical University Affiliated Fuxing Hospital（ 18 normal menstruation, 14 perimenopause, 13 postmenopause）, the expression of AQP2 mRNA was detected by Real -time PCR. Univariate analysis and logistic regression were used for analysis. Results：AQP2 mRNA was found in hECE. The expression of AQP2 mRNA was significantly higher in child- bearing period than those in peri-menopausal and menopausal periods（ P 〈0.05） ; while there was no difference between perimenopausal and menopausal group （P〉0.05）. The expression of AQP2 mRNA and age, plasma osmotic pressure were negatively correlated （ P 〈0.05）, while AQP2mRNA and estrogen were posi- tively correlated （ P 〈 0.05 ）. Conclusions. Reduced expression of AQP2 mRNA in peri-menopausal and menopausal periods will cause less vaginal secretion, which might be the reason of dryness of vagina.