子宫内膜异位症患者在位子宫内膜神经纤维分布的研究

Nerve Fibres Distribution in Eutopic Endometrium of Women with Endometriosis

目的:研究子宫内膜异位症(EMT)患者的在位子宫内膜的功能层中神经纤维的分布状况。方法:选取2006年1月至2008年12月以痛经为主诉并行子宫切除的EMT患者25例为研究组,随机选取同期无此疾病的行子宫切除的患者40例作为对照组,利用免疫组织化学技术,使用多克隆家兔抗人抗蛋白基因产物9.5染色标记子宫在位内膜的神经纤维,用数码相机照相,分析其神经纤维密度。结果:在EMT患者子宫内膜的功能层中出现神经纤维,而非EMT患者子宫内膜的功能层中未出现神经纤维(P<0.01)。结论:EMT患者子宫内膜的功能层中神经纤维异常增多,可能与EMT患者的疼痛症状有关。

Objective：Nerve fibres distribution in the functional layer of endometrium of women with endometriosis was investigated. Methods：Histological sections of endometdal tissue were prepared from endometfial curettings and hysterectomies performed on women with endometriosis （ n = 25 ） and without endometriosis （ n =40）. Immunohistochemistry was used to detect nerve fibres by highly specific polyclonal rabbit antibody PGP 9.5. The assessment of nerve fibre density was performed by Image Pro Plus Discovery. Results： Nerve fibres were identified throughout the functional layers of the endometrium in all endometriosis patients, but not found in the functional layer of the endometrium in women without endometriosis（ P 〈 0.01 ）. Conclusions.Nerve fibres detected in the functional layer in all women with endometriosis may have important implications for understanding the generation of pain in these patients.