SBA-50型葡萄糖生物传感分析仪测定麦芽糖的研究

Study on Determination of Maltose by Using SBA_50 Glucose Biosensor

本文研究了用ＳＢＡ＿５０型葡萄糖生物传感分析仪测定麦芽糖的方法。麦芽糖的测定范围为０～２００ｍｇｄＬ，测定回收率平均为９９６９％。将麦芽糖溶液中加一定量的葡萄糖糖化酶（３００ｕｍＬ底物），６０℃水解５ｍｉｎ，取水解液２５μＬ，注入ＳＢＡ＿５０型葡萄糖生物传感分析仪中，２０ｓ即可得到结果。该方法简便、快速、准确度高。

Determination of maltose using SBA_50 Glucose Biosensor was studied.The range of maltose determination is 0～200 mgdL and the average recovery rate is 99.69%. Maltose can be measured by SBA_50 Glucose Biosensor in 20 seconds after hydrolyzed with golucoamylase at 60℃ for 5 minutes. This method has advantages of simplicity, rapidity and high accuracy.