干燥综合征患者细胞因子途径、Jak-Stat信号途径以及神经激活受体配体途径相关基因表达的初步分析

Gene Expression of Cytokine Pathway,Jak-Stat Signal Pathway and Neuroactive Ligand-receptor Pathway in Patients with Primary Sjgren′s Syndrome.

目的分析干燥综合征患者的细胞因子途径、Jak-Stat信号途径及神经激活受体配体途径相关基因的表达情况并分析意义。方法取3例干燥综合征患者及3例健康对照者的外周血单个核细胞,提取总RNA反转录至cDNA后,分别用Cy5及Cy3荧光标记干燥综合征组及对照组的cDNA样品,与寡核苷酸基因芯片杂交。采用图像分析软件提取芯片图像数据,以Cy5/Cy3的比值(SAM软件)寻找差异表达基因,并进一步用分子功能注释软件(MAS系统)进行处理。结果干燥综合征患者细胞因子途径、Jak-Stat信号途径及神经激活受体配体作用途径相关基因的差异表达有统计学意义(P<0.05)。以上途径中基因PF4、GZMA表达下调,基因IL-2RA、IL-10表达上调。结论干燥综合征的发病可能是多基因调控异常所致,细胞因子途径、Jak-Stat信号途径及神经激活受体配体途径的差异表达基因可能为研究该病的发病机制及新的治疗靶向提供了重要依据。

Objective This study is to investigate cytokine pathway, Jak - Stat signal pathway, neuroactive ligand - receptor pathway gene expression pattern of peripheral blood mononuelear cell （PBMC） of primary sjogren syndrome patients. Methods The PBMC sample of 3 patients with sjogren syndrome and 3 healthy volunteers with consistent age were collected. The total RNAs was extracted from the PBMC samples, and reverse transcripted in vitro transcription （IVT） , labeled with Cy5/Cy3 and hybridized on the gene chips. After scanning and data extraction with LuxSean 3.0, differentially expressed genes were analyzed with SAM method. The online tool of molecule analysis system （MAS） was used for biological knowledge mining. Results Statistical difference was calculated between the patient and control group in the following three pathways ： cytokine pathway, Jak - Stat signal pathway, neuroactive ligand - receptor pathway. A- mong these, genes of IL - 2RA, IL - 10 were up - regulated and genes of PF4, GZMA were down - regulated. Conclusion Understanding of differently expressed genes should help us disclose the potential molecular mechanism underlying the development process of pathogenesis of primary Sjogren＇s syndrome. And the research may provide new target therapy for SS.