蛋白质组学方法筛选早期肺鳞癌相关蛋白

Proteomic screening of the proteins associated with early lung squamous carcinoma

目的:应用蛋白质组学技术筛选早期肺癌癌变相关蛋白。方法:利用双向凝胶电泳方法分离人早期肺鳞癌组织和相应癌旁正常组织总蛋白,选择差异蛋白质点进行质谱分析。免疫组织化学法验证部分差异蛋白质的表达差异。结果:肺鳞癌组织与癌旁正常组织的双向凝胶电泳图谱中平均蛋白质点数分别为626和602个。选择在癌组织中高表达的10个差异蛋白点进行质谱分析,最终鉴定为膜联蛋白1(annexin-1,Anx-A1)、热休克蛋白27(heat shock protein 27,HSP27)等与细胞周期、信号转导等功能相关的蛋白质。免疫组织化学检测结果显示,Anx-A1和HSP27蛋白在肺鳞癌组织中的阳性表达率均显著增高(P<0.05)。结论:蛋白质组学方法是一种应用于初步筛选早期肺癌相关蛋白的有效方法,所鉴定的蛋白为进一步筛选用于肺癌早期诊断及其治疗的分子标志物奠定了前期基础。

Objective：To screen the early biomarkers associated with lung carcinoma by using proteomic method. Methods：The proteins of early lung squamous cell carcinoma tissues and adjacent normal tissues were separated by two-dimensional gel electrophoresis （2-DE） and differentially expressed proteins were screened by mass spectrometry （MS）. The differential expressions of part proteins were verified by immunohistochemical method. Results：The proteomic profiles of lung squamous cell carcinoma and the adjacent normal tissues were established and the average matched protein spots in the two group were 626 and 602,respectively. Ten protein spots with higher expression in lung squamous cell carcinoma tissues were identified as Annexin 1（Anx-A1）,heat shock proteins （HSP27）,etc. The proteins were mainly related with cell cycle and signal transduction. The results of immunohistochemistry showed that the positive expression rates of Anx-A1 and HSP27 in lung squamous cell carcinoma tissues were significantly increased （P〈0.05）. Conclusion：Proteomic technology was an effective method for preliminary identification of the proteins associated with early lung carcinoma. The identified proteins laid a solid foundation for further screening the molecular markers for early diagnosis and treatment of lung cancer.