摘要
目的比较以CD105和CD34为血管内皮细胞标记物在早期直肠癌新生血管中表达的差异,探讨CD105-微血管密度(MVD)作为Ⅰ-Ⅱ期直肠癌预后预测指标的可能性。方法分别应用抗CD105、CD34抗体对380例Ⅰ-Ⅱ期直肠癌组织微血管进行免疫组织化学标记,多因素回归分析CD105-MVD、CD34-MVD与早期直肠癌肝转移及预后的相关性。结果CD105在直肠癌新生微血管中特异性表达,在正常组织的血管无表达,而CD34在肿瘤及正常组织的大小血管中均广泛表达,两者表达率有显著性差异(P<0.001);CD105-MVD与肿瘤浸润深度、病理组织类型、肝转移率呈正相关。多因素分析表明CD105-MVD是影响全组直肠癌预后的独立因素,CD105-MVD高表达时患者死亡的危险度是低表达患者的3.032倍(95%CI=1.481-6.804)。而CD34-MVD与直肠癌的预后无明显相关性。结论CD105是一种较CD34更有特异性的肿瘤性血管内皮细胞标记物,CD105抗体标记的肿瘤微血管密度可作为预测早期直肠癌预后的重要分子生物学指标。
Objective To investigate the difference and significance of CD105 and CD34 in detecting new born blood vessels in rectal carcinoma at early stage.Methods Tumor tissues from 380 potentially resected rectal cancer patients in pathological stages Ⅰ-Ⅱ were analyzed for MVD by immunohistochemical S-P method with anti CD105 and CD 34 antibody respectively.Logistic analysis was used to detect the relevance of CD105-MVD,CD34-MVD and liver metastases,prognosis in early rectal carcinoma.Results Newborn blood vessels and normal blood vessels could be clearly distinguished in CD105 stained slides,but not in CD34 stained slides.The detection of MVD marked by CD34 was obviously higher than that marked by CD105 in rectal carcinoma.Multivariate analysis revealed that CD105-MDV but not CD34-MDV was a independent indicator to predict probability of hepatic metastasis and poor prognosis of rectal carcinoma with phaseⅠ-Ⅱ.The cases positive for CD105-MVD had a significantly hazard ratio （95%CI） for death from early rectal carcinoma for 3.032 （1.481-6.804）.Conclusion CD105 is more suitable than CD34 in studying the newborn blood vessels of rectal cancer.Detecting the expression of CD105-MVD may help to predict clinical outcome and design further individualized intensive adjuvant treatment.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第2期111-116,共6页
Chinese Journal of Gastroenterology and Hepatology
基金
福建省自然基金资助项目(2009J01147)
