子癎前期患者血清对人早孕细胞滋养细胞活力和浸润功能的影响

Effects of preeclampsia serum on activity and invasive ability of cultured cytotrophoblasts of first trimester of pregnancy

目的探讨子癎前期患者血清对人早孕细胞滋养细胞活力和浸润功能的影响。方法采用组织块贴壁法培养人早孕期(孕6～8周)细胞滋养细胞,传代后待细胞长满至70%～80%,分别加人正常孕妇血清(正常组)和子癎前期患者血清(子癎前期组)培养24 h;CCK-8法测定细胞活力;Transwell技术检测细胞滋养细胞的浸润功能;RT-PCR法检测滋养细胞MMP-2、MMP-9和PAI-1 mRNA的表达。结果子癎前期组细胞滋养细胞活力和浸润能力均低于正常组(P<0.05和P<0.01)。与正常组比较,子癎前期组细胞滋养细胞MMP-2和MMP-9 mRNA的表达明显下降,而PAI-1 mRNA的表达明显升高,差异均有统计学意义(P<0.01)。正常组和子癎前期组中,MMP-2 mRNA与PAI-1 mRNA的表达均呈负相关(r=-0.985,P<0.01;r=-0.933,P<0.05);MMP-9 mRNA与PAI-1 mRNA的表达无相关性。结论子癎前期患者血清可能通过调节细胞滋养细胞中MMP-2、MMP-9和PAI-1的表达而影响细胞的浸润能力。

Objective To investigate the effects of preeclampsia serum on activity and invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Methods Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by tissue explants adherent method, and were incubated with serum of women with normal pregnancy （normal group） and preeclampsia （preeclampsia group）, respectively for 24 h. The activity of cytotrophoblasts was examined by CCK-8, invasive ability was determined by Transwell invasion assay, and expression of MMP-2, MMP-9 and PAI-1 mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results The activity and invasive ability of cytotrephoblasts in preeclampsia group were lower than those in normal group （ P 〈 0.05, P 〈 0.01 ）. Compared with normal group, the expression of MMP-2 and MMP-9 mRNA of cytotrophoblasts was significantly lower （P 〈 0.01）, and the expression of PAI-1 mRNA was significantly higher （P 〈 0.01）. In both groups, the expression of MMP-2 mRNA was negatively related to that of PAI-1 mRNA （r = - 0. 985, P 〈 0.01; r = - 0.933, P 〈0.05）, while there was no correlationship between the expression of MMP-9 mRNA and that of PAI-1 mRNA. Conclusion The preeclampsia serum may affect the invasive ability of cytotrophoblasts by regulating the expression of MMP-2, MMP-9 and PAI-1.