原发性肝癌组织中RANTES的表达与微血管密度的关系

Relationship between expression of RANTES and microvascular density in tissues of primary hepatocarcinoma

目的研究原发性肝癌(PHC)组织中受激活调节正常T细胞表达和分泌因子(RANTES)的表达和微血管密度(MVD)的关系,并探讨其临床意义。方法选择47例PHC癌组织和癌旁组织标本,免疫组织化学法检测组织中RANTES的表达并计算MVD,分析RANTES表达、MVD与PHC临床病理特征的关系,并探讨两者的相关性。结果癌组织中RANTES的阳性表达率和表达评分均显著高于癌旁组织(55.32%vs 19.15%、1.89±1.77 vs 0.77±1.29)(均P<0.01);癌组织中MVD显著高于癌旁组织(67.30±13.68 vs 37.20±10.58)(P<0.01);除伴转移病例癌组织中MVD高于无转移病例[(73.50±13.77)/HP vs(64.10±12.68)/HP](P<0.05)外,RANTES表达、MVD与PHC的其他病理特征无明显关系。相关性分析显示,癌组织中RANTES表达评分与MVD呈正相关(r=0.386,P<0.05)。结论RANTES可能与PHC的血管生成有密切关系。

Objective To investigate the relationship between the expression of regulated-upon activation normal T expressed and secreted （RANTES） and microvascular density （MVD） in hepatocarcinoma （PHC） tissues, and explore its clinical significance. Methods The samples of PHC tissues and adjacent tissues in 47 patients were collected. The expression of RANTES in tissues was detected by immunohistochemistry, and MVD was calculated. The relationship among the expression of RANTES, MVD and clinicopathological features was analysed. Results The positive expression rate and expression score of RANTES in PHC tissues were significantly higher than those in adjacent tissues （55.32% vs 19.15%, P 〈0.01; 1.89 ± 1.77 vs 0. 77 ± 1.29, P 〈 0.01）. MVD in PHC tissues was significantly higher than that in adjacent tissues （67.30 ± 13.68 vs 37.20 ± 10.58, P 〈 0.01）. MVD of PHC tissues in patients with metastasis was significantly higher than that in patients without metastasis [（73.50 ± 13.77）/HP vs （64.10 ± 12.68）/HP, P 〈0.05], while there were no correlations among MVD, expression of RANTES and the other clinieopathological features of PHC. MVD was positively correlated with the expression score of RANTES in PHC tissues （ r = 0. 386, P 〈 0.05）. Conclusion RANTES might be closely related to the angiogenesis in PHC.