α-三噻吩对白纹伊蚊幼虫蛋白质、酯酶及脂质过氧化的影响

Effect of Alpha-terthienyl on Protein,Esterase and Lipid Peroxidation of Aedes albopictus Larvae

目的研究α-三噻吩(α-T)对白纹伊蚊幼虫蛋白质含量、酯酶活力及脂质过氧化反应等的影响。方法采用敏感株和抗性株白纹伊蚊Ⅳ龄幼虫实验,按照Bradford法测定其蛋白质含量,实验组饲养液中含5.34μg/Lα-T,对照组饲养液中用丙酮(3.95μg/L)代替α-T;用组织化学方法测定酯酶活力,实验组饲养液中α-T浓度为6.24μg/L,对照组饲养液中不含α-T。按照TAB法测定丙二醛(MDA)含量,将敏感株白纹伊蚊Ⅳ龄幼虫分5组:A组为丙酮对照组,饲养液中丙酮浓度为3.95μg/L,不含α-T;B组为紫外光照(UV)对照组,饲养液同A组;C、D、E组为实验组,饲养液中α-T浓度分别为4.58、5.34和6.24μg/L。以上各组蚊幼虫均先在黑暗中饲养1h,经紫外光照射1h(A组不照射)转为常规饲养。结果α-T与紫外光共同作用下蚊幼虫蛋白质含量,敏感株实验组(1.225mg/ml)显著高于对照组(1.120mg/ml)(P<0.05),抗性株实验组(1.199mg/ml)亦显著高于对照组(1.114mg/ml)(P<0.05)。α-T与UV共同作用2、4、6和8h,敏感株、抗性株的实验组酯酶活力均比其对照组低,其中实验组作用8h酯酶活性最低(P<0.05)。敏感株丙酮对照组、紫外光对照组和3个实验组的MDA含量分别为2.286、2.322、3.156、4.188和4.684nmol/mg蛋白,且MDA含量与α-T浓度呈正相关(P<0.05)。结论在紫外光作用下,α-T能增加白纹伊蚊幼虫蛋白质含量和MDA含量,抑制其酯酶活力。

[Abstract] Objective To study the effect of alpha-terthienyl（α-T） on protein, esterase and lipid peroxidation of Aedes albopictus larvae. Methods Sensitive and resistant strains of Aedes albopictus stage IV larvae were used. Bradford method was used to detect protein content. The breeding fluid of experiment group contained α-T （5.34μg/L）, and control group contained only acetone（3.95 μg/L）. Histochemistry method was used to detect esterase activity. Larvae in the experiment group were cultured in fluid containing α-T（6.24 μg/L） but no α-T in the control group. TBA method was used to detect malondialdehyde （MDA）. Larvae of the sensitive strain were divided into 5 sub-groups： A-acetone control （containing acetone 3.95 μg/L）, B-ultra-violet irradiation （UV） control （same with A but treated by UV）, C, D, E- experiment groups with α-T （4.58, 5.34 and 6.24 μg/L respectively）. All groups were kept in dark condition for 1 hour, followed by UV for 1 hour （except group A）, then fed under normal condition. Results With UV and α-T, the protein content of experiment group （1.225 mg/ml） was higher than that of control （1.120 mg/ml） （P〈0.05） in sensitive strain; that of experiment group （1.199 mg/ml） was higher than that of control （1.114 mg/ml） （P〈0.05） in the resistant strain. After 2, 4, 6, and 8 hour treated by both α-T and UV, the esterase activity all decreased in experiment group, and reached to the lowest 8 hours later （P〈0.05）. MDA contents was 2.286 nmol/mg protein in acetone control group and 2.322 nmol/mg protein in UV control, but 3.156, 4.188 and 4.684 nmol/mg protein respectively in the 3 experiment groups after treated by α-T and UV. The higher dose of α-T, the higher content of MDA （P〈0.05）. Conclusion Under UV,α-T can increase the protein and MDA content of the larvae of Ae. albopictus but decrease the esterase activity.