中药复方祛毒增宁胶囊抗艾滋病毒体外药效学的研究

In vitro pharmacodynamics study of an anti-HIV Chinese herbal formulation

本研究以现代医学为基础,结合中医辨症的原则,特别是经过体外抗病毒实验的筛选,选择既具有抗病毒作用,可增强机体免疫功能又符合中医辨症原则,以黄芩等4味中药组成复方,经过正交设计,优选提取溶剂和工艺,制成胶囊,暂定名为祛毒增宁胶囊(ZL-1)。体外药效学研究表明,祛毒增宁胶囊可有效抑制病毒在MT4、HeLa和PBMC中的复制,IC50分别为105.2、70.7及77.4μg·mL-1。祛毒增宁胶囊中主要成分JH与齐多夫定(AZT)不同剂量配伍,表现出明显的增效作用,同时,对蛋白酶抑制剂抗性株也有明显的抑制作用,在0.12mg·mL-1质量浓度下抑制率为100%。此制剂已完成临床前研究,并获批准进入临床观察阶段。

AIDS caused by HIV-1,is a major threat to human being.An anti-HIV formulation from Chinese herbs,so called ＂Qu Du Zeng Ning＂,have been recently developed.In this work,the pharmacodynamics of the formulation in vitro was studied.The results showed that Qu Du Zeng Ning inhibit the replication of HIV-1 efficiently in all cell-based assay,with IC 50 at 105.2,70.7,77.4 μg·mL-1,separately.A significant synergy between the formulation and zidovudine （AZT） was observed,and it also showed a potent activity against HIV-1 drug-resistant mutant.