美洲黑杨×小叶杨杂种多倍体诱导研究

Polyploid Induction on the Hybrid of Populus deltoides×Populus simonii

用不同浓度秋水仙素处理美洲黑杨(Populus deltoides)与小叶杨(Populus simonii)杂种后代以诱导产生多倍体,并采用流式细胞术(FCM)对初选的多倍体材料进行DNA含量分析。结果表明:小于0.1%秋水仙素浓度处理对诱导多倍体无作用;0.3%及其以上浓度处理对诱导产生多倍体效果较好,但随处理浓度增加秋水仙素对杨树的毒害作用增大,0.5%浓度处理可使顶芽死亡率高达71.43%,且在顶芽死亡的植株中未发现多倍体单株。应用气孔性状对388株扦插苗进行多倍体初选后,采用流式细胞术对初选的5株拟多倍体进行DNA含量分析,鉴定到2株四倍体材料。获得的四倍体材料与二倍体植株在株高和叶绿素含量上差异显著,但在SOD和POD活性上无显著性差异。

The hybrid from the cross of Populus deltoides and Populus simonii was treated with different concentrations of colchicine solutions.The DNA content of the elementary filtrated plants was analyzed by flow cytometry（FCM）.The results as follow：The toxic effect to poplar was getting more and more serious with the increase of colchicine concentration,but no polyploid plants were found for those plants whose terminal bud were died.According to the characters of stomata,elementary filtration of the polyploid was carried out from 338 plants.DNA content analysis with flow cytometry was used to check the ployploid,and 2 tetraploid plants were obtained.Tetraploid was different from diploid in plant size and cholorophyll contents,but the SOD and POD activities were not significantly different.