期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

STAT3靶向短发夹RNA干扰重组载体对结肠癌SW480细胞的沉默作用 被引量：1

下载PDF
导出
摘要 目的探讨STAT3基因短发夹RNA(shRNA)表达质粒对结肠癌SW480细胞STAT3基因的干扰作用。方法根据siRNA设计原则,构建靶向STAT3基因的短发夹RNA干扰质粒(pGPU6/GFP/STAT3),使用脂质体法转染人结肠癌细胞系(SW480),通过RT-PCR和Western印迹检测结肠癌SW480细胞STAT3基因mRNA和蛋白表达水平。结果pGPU6/GFP/STAT3重组质粒经限制性内切酶酶切及测序证明基因插入正确。质粒成功转染SW480细胞后,该细胞的STAT3mRNA和蛋白表达明显下降(P<0.05)。结论成功构建靶向STAT3基因的shRNA表达载体,转染SW480细胞,能有效抑制细胞的STAT3mRNA和蛋白表达,为STAT3基因靶向治疗提供前期的实验依据。
作者 王朝阳 崔春晖 姚晓军 黄宗海
机构地区 南方医科大学珠江医院普外科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第3期355-358,共4页 Chinese Journal of Gerontology
关键词 结肠癌 STAT3 RNA干扰 基因表达
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献13

  • 1Xiong H,Zhang ZG,Tian XQ,et al. Inhibition of JAK1,2/STAT3 signaling induces apoptosis, cell cycle arrest, and reduces tumor cell invasion in colorectal cancer cells[J]. Neoplasia,2008 ; 10(3 ) :287-97.
  • 2Klampfer L The role of signal transducers and activators of transcription in colon cancer[ J ]. Front Bio Sci ,2008 ; 1 ( 13 ) :2888-99.
  • 3Chen Z, Han ZC. STAT3 : a critical transcription activator in angiogenesis[J]. Med Res Rev,2008 ;28 ( 2 ) : 185 -200.
  • 4Barboza JA, Wang S, Schaefer TS. Generation and characterization of a constitutively active Star3 protein [J]. Mol Biol Rep, 2004 ; 31 ( 1 ) : 13- 21.
  • 5Shi CS, Kehrl JH. Pyk2 amplifies epidermal growth factor and c-Src-induced Star3 activation[J]. J Biol Chem,2004;279(17) :17224-31.
  • 6Snyder M, Huang XY,Zhang JJ. Identification of novel direct Stat3 target genes for control of growth and differentiation[J]. J Biol Chem,2008 ;283 (7) :3791-8.
  • 7马向涛,王杉,叶颖江,社如昱,崔志荣.Stat3及其靶基因产物与结直肠癌恶性程度关系的研究[J].癌症,2003,22(11):1135-1139. 被引量：31
  • 8吴健,王方金,何蕴韶.STAT3特异性小干扰RNA表达载体的构建及其对STAT3表达的抑制[J].肿瘤,2005,25(1):37-40. 被引量：8
  • 9Rawlings JS, Rosier KM, Harrison DA, et al. The JAK/STAT signaling pathway [J]. J Cell Sci,2004 ; 117 (8) : 1281.
  • 10Bromberg JF, Wrzeszczymska MH, Devgan G,et al. Star3 as an oncogene [J]. Cell, 1999 ;98 (3) :295-303.

二级参考文献34

  • 1Bowman T, Garcia R, Turkson J, et al. STATs in oncogeoesis [J]. Oncogene, 2000, 19(21): 2474 - 2488.
  • 2Levy DE, Damell JE Jr. Stats: transcriptional control and biological impact[J]. Nat Rev Mol Cell Biol, 2002, 3(9):651 - 662.
  • 3Catlett-Falcone R, Landowski TH, Oshiro MM, et al. Constitutive activation of Stat3 signaling confers resistance to apoptosis in human U266 myeloma cells[J]. Immunity, 1999,10(1): 105-115.
  • 4Grandis JR, Drenning SD, Zeng Q, et al. Constitutive activation of Stat3 signaling abrogates apoptosis in squamous cell carcinogenesis in vivo[J]. Proc Natl Acad Sci U S A, 2000, 97 (8) : 4227 - 4232.
  • 5Real PJ, Sierra A, De Juan A, et al. Resistance to chemotherapy via Stat3-dependent overexpression of Bcl-2 in metastatic breast cancer cells[J]. Oncogene, 2002, 21 (50):7611 -7615.
  • 6Dhir R, Ni Z, Lou W, et al. Stat3 activation in prostatic carcinomas[J]. Prostate, 2002, 51 (4): 241 - 246.
  • 7Wang S, Evers BM. Caco-2 cell differentiation is associated with a decrease in Stat protein levels and binding[J]. J Gastrointest Surg, 1999, 3(2): 200 -207.
  • 8Masuda M, Suzui M, Yasumatu R, et al. Constitutive activation of signal transducers and activators of transcription 3 correlates with cyclin D 1 overexpression and may provide a novel prognostic marker in head and neck squamous cell carcinoma[J]. Cancer Res, 2002, 62(12): 3351-3355.
  • 9Bromberg JF, Wrzeszczynska MH, Devgan G, et al. Stat3 as an oncogene[J]. Cell, 1999, 98(3): 295 - 303.
  • 10Huang M, Page C, Reynolds RK, et al. Constitutive activation of stat3 oncogene product in human ovarian carcinoma cells[J]. Gynecol Oncol, 2000, 79 ( 1 ) : 67 - 73.

共引文献40

同被引文献4

  • 1Archana Thakur RNA interference revolution[J].Molecu-lar Biology and Genetics.2003,6(1):39.
  • 2Wurzer WJ,Planz O,Ehrhardt C,et al.Caspase-3 activa-tion is essential for efficient influencenza virus propagation[J].EMBoj,2003,22(11):2717.
  • 3Zender L,Hutker S,Liedtke C,et al.Caspase-8 small in-terfering RNA prevents acute liver failure in mice[J].Proc Nail Acad Sci USA,2003,100(70):7797.
  • 4袁成良,金晓岚.RNA干扰原理及应用研究进展[J].西部医学,2009,21(4):664-666. 被引量：6

引证文献1

中国老年学杂志
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部