老龄大鼠脑组织内不同部位葡萄糖转运蛋白3的表达

Expression of GLUT 3 in different brain regions of aged rats

目的:观察老龄大鼠的脑组织内不同部位的葡萄糖转运蛋白3(GLUT 3)的表达情况,探讨GLUT 3在神经系统衰老过程中的作用。方法:用免疫组织化学染色方法,测定3月龄、18月龄及30月龄大鼠(各10只)脑组织内不同部位GLUT 3的表达强弱。结果:各月龄组大鼠脑组织内不同部位阳性细胞数均有显著差异(P<0.01),其中海马区,尤其是海马CA1区阳性细胞数最多,而运动皮质区及第1小脑小叶阳性细胞数相对较少。结论:在老龄大鼠脑组织中GLUT 3的表达减弱,提示GLUT 3在神经系统衰老过程中发挥一定作用。

Objective： To examine the distribution of glucose transport 3（GLUT 3） in different brain regions of aged rats and to investigate its role in ageing process of the nervous system.Methods： The GLUT 3 expression in different brain regions was examined with immunohistochemical method in rats aged 3,18 and 30 months,respectively.Results： The number of GLUT 3-positive cells varied in the different brain regions in rats of all age groups（P0.01）;the CA1 region contained the greatest number of positive cells,and fewer in the motor cortex and cerebellum.The number of GLUT 3-positive cells was reduced in the brain of aged rats（P0.01）;and the neural cells in 4 different brain regions presented with large cell body and loose alignment.Conclusion： The expression of GLUT 3 decreased in aged rats,which suggests that GLUT 3 may be involved in the ageing process of nervous system.