脑梗死载脂蛋白E基因多态性与气虚痰热证的关系

Relationship Between Apolipoprotein E Gene Polymorphism and Syndrome of Qi Deficie-ncy and PhlegmHeat in Patients with Cerebral Infarction

目的:研究脑梗死载脂蛋白E(ApoE)基因多态性与中医气虚痰热证候的关系。方法:运用聚合酶链反应-限制性片段多态性(PCR-RFLP)技术检测50例脑梗死气虚痰热证患者及50例健康对照者的ApoE基因多态性分布特征,并进行比较分析。结果:共发现6种基因型,分别为E2/2、E2/3、E2/4、E3/3、E3/4、E4/4。脑梗死气虚痰热证患者ApoE3/4基因型和ε4等位基因频率均高于健康对照组,ε2等位基因频率低于健康对照组,两组比较差异具有统计学意义(P<0.05)。结论:ApoE基因多态性与脑梗死气虚痰热证有一定关系,其中E3/4基因型与气虚痰热证有一定关联。ε4等位基因可能是其易感基因和风险因子,ε2等位基因可能是其保护因子。

Objective：To investigate the relationship between apolipoprotein E gene polymerphism and syndrome of qi deficiency and phlegmheat in patients with cerebral infarction.Methods：We included 50 patients of cerebral infarction with the syndrome of qi deficiency and phlegmheat as well as 50 health control patients.ApoE gene polymorphism was evaulated in both groups using PCR-RFLP technique.Results：6 genotypes were detected：E2/2、E2/3、E2/4、E3/3、E3/4、E4/4,The frequencies of the ApoE3/4 genotype and the ε4 allele in the cerebral infarction patients with the syndrome of qi deficiency and phlegmheat werehigher than those in the health control subjects,while the ε2 allele frequence was lower,with significant statistical difference（P0.05）in both comparisons.Conclusion：Apolipoprotein E gene polymorphism was correlated to the syndrome of qi deficiency and phlegmheat in patients with cerebral infarction,so does e3/4 genotype.While ε4 allele might the predisposing genes or the risk factor,and which indicated that the ε2 allele might acted as a protective factor.