期刊文献+

肺气虚模型大鼠结肠水通道蛋白2表达变化及机制研究 被引量:7

Mechanism Study of Colon Aquaporin2 Expression in Lung-Qi Deficiency Model Rats
下载PDF
导出
摘要 目的:观察肺气虚模型大鼠结肠组织AQP2表达的变化及肺组织、血浆中ET、IL-1β、TNF-α含量变化。为肺与大肠相表里理论提供依据。方法:大鼠20只随机分为模型组和对照组,应用免疫组化方法观察AQP2蛋白含量的变化,应用放免法观察肺组织、血浆中ET、IL-1β、TNF-α含量变化。结果:肺气虚模型组结肠组织AQP2表达低于正常组(P<0.05);肺组织、血浆中ET、IL-1β、TNF-α含量高于正常组(P<0.05)。结论:肺气虚模型大鼠结肠组织AQP2表达减少,肺组织、血浆中ET、IL-1β、TNF-α含量增加。提示肺主"宣降"功能与大肠濡润功能关系的科学内涵,为中医学肺与大肠相表里基本理论提供实验依据。 Objective:To provide experimental evidence for the tradi-tional Chinese medical theory of "lung being connected with large intestine".The effects of colon aquaporin-2 expression and the change of ET,IL-1β,TNF-αin blood and lung tissues in Lung-Qi deficiency model rats was observed.Methods:Twenty healthy rats were divided into the model group and the control group.The method of Immunohisto-chemistry was used to observe the expression of aquaporin-2 in kidney.Radioimmuno-assay was applied to measure the change of ET,IL-1β,TNF-αin blood and lung tissues.Results:The expression of aquaporin-2 in colon was lower in model group than in the control group.ET,IL-1β,TNF-α concentrations of blood and lung tissue in the model group were markedly higher than those in the control group(P0.05).Conclusion:The expression of aquaporin-2 in colon was lower but ET,IL-1βand TNF-α concentrations of blood and lung tissue was higher in Lung-Qi deficiency model rats.One of the substance basis of "lung being connected with large intestine" may be the change of AQP2 expression in the colon tissues.
机构地区 辽宁中医药大学
出处 《辽宁中医杂志》 CAS 北大核心 2010年第2期353-355,共3页 Liaoning Journal of Traditional Chinese Medicine
基金 辽宁省教育厅基金资助项目(2008Z219) 辽宁省自然科学基金资助项目(20092032)
关键词 肺气虚 结肠AQP2 ET IL-1Β TNF-Α lung-Qi deficiency colon aquaporin2 ET IL-1β TNF-α
  • 相关文献

参考文献10

二级参考文献72

共引文献185

同被引文献158

引证文献7

二级引证文献32

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部