Cyr61在人肿瘤组织中的表达

Cyr61 expression in several human tumor tissues

目的观察分析富含半胱氨酸61(cysteine-rich61,Cyr61)在人类肿瘤组织中的表达情况。方法采用HE染色、免疫组化的方法检测多组织肿瘤组织芯片和30例肿瘤组织标本(包括食管癌、胃癌、直肠癌、肺癌、乳腺癌和肝癌组织标本各5例)中Cyr61的表达。结果组织芯片中79%(38/48)的肿瘤组织Cyr61表达阳性,93%(28/30)肿瘤组织标本中Cyr61表达阳性。其中神经、食管和甲状腺等肿瘤组织中呈强阳性表达,胃、肝、结肠、直肠、肺、肾、子宫、皮肤、卵巢、乳腺等组织的肿瘤呈阳性表达,在膀胱、前列腺和胰腺等肿瘤组织中表达较弱。结论Cyr61在多数肿瘤组织中均过表达,在不同肿瘤组织中表达程度不同,Cyr61可能成为新的肿瘤标志物应用于多种肿瘤的诊断。

Objective To observe and analyze the expression of cysteine-rich61 （Cyr61）, a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene, in a variety of human tumors, in order to explore its role as a new marker for tumor diagnosis. Methods HE and immuno- histoehemieal staining were used to detect the expression of Cyr61 in several organ tumor tissue array and 30 human tumors samples. Results The expression of Cyr61 was found in human tumor tissue array and in human tumor samples with positive straining in 79% （38/48） and 93% （28/30）, respectively. Strong positive staining was found in the tumors from the brain, esophagus and thyroid. Positive staining was seen in the tumors from the stomach, liver, colon, rectum, lungs, kidneys, uterine, skin, ovary and breasts. Negative staining was defined in the tumors from the prostate, bladder and pancreas. Conclusion Overexpression of Cyr61 is seen in many human tumors and the expression is different in different types of tumors. Cyr61 is probably used to diagnose the tumor as a new marker.