摘要
利用RT-PCR从欧美杨107次生木质部中克隆出一709bp的hct基因片段,其开放阅读框为708bp,编码236个氨基酸,具有HCT共有的DFGWG序列和HXXXQ活性位点,拟表达蛋白含有一个二硫键,属于亲水性稳定型蛋白。克隆片段拟表达蛋白质没有信号肽,也没有跨膜结构域,包含α-螺旋区和少量β-折叠,具有比较多的无规则卷曲。
One 709bp hot gene was amplified from secondary xylem in poplar by RT-PCR, containing a 708bp ORF（open reading flame）which encoded 236 amino acids. The predict protein had the DFGWG sequence and HXXXQ motif common in HCT, and contained a disulfide bond, belonged to stable protein of hydropathicity. There was no signal peptide and trans-membrane domain, containing α-helices, a little β-strands and large number of random coils.
出处
《辽宁林业科技》
2010年第1期12-15,33,共5页
Liaoning Forestry Science and Technology
基金
辽宁省自然科学基金(20062146)
关键词
HCT
序列分析
蛋白质结构预测
shikimate/quinate hydroxycirmamoyltransferase
sequence analysis
structure prediction
