肾清饮对糖尿病大鼠肾脏的保护作用及其可能的机制

Protective effect of Shenqingyin Decoction on the kidney of experimental diabetic rats and its mechanism

目的探讨肾清饮对糖尿病大鼠肾脏的保护作用及可能的机制。方法40只链脲佐菌素诱导的糖尿病大鼠随机分成模型组、肾清饮组、贝那普利组和肾清饮联合贝那普利组,连续给药8周后观察血糖、肾功能和24 h尿蛋白定量的变化,分别检测肾组织及尿中丙二醛(MDA)含量,肾组织中超氧化物岐化酶(SOD)的活性,血清转化生长因子(TGF-β1)水平的变化,光镜观察肾脏病理形态学的改变。结果各药物组肾功能明显改善,与对照组比较无显著性差异(P>0.05),但对血糖无明显改善作用,24 h尿蛋白量较模型组显著下降(P<0.05或P<0.01),联合组下降最为显著(P<0.01);各药物组肾组织及尿MDA含量、肾组织SOD活性和血清TGF-β1表达与模型组有显著性差异(P<0.05或P<0.01),联合组与其他药物组比较差异显著(P<0.05)。光镜观察各药物组肾脏病理改变均有不同程度改善,各组间无明显差异。结论肾清饮对糖尿病大鼠肾脏有保护作用,与贝那普利联用有协同作用。其作用机制可能与抑制氧化应激和增强机体抗氧化能力,降低炎症因子TGF-β1的表达有关。

Objective To investigate the possible protective effect of Shenqingyin Decoction on the kidney of experimental diabetic rats and its possible mechanism.Methods Totally 40 rats with diabetes induced by streptozocin were divided into 4 groups： diabetes model group,Shenqingyin group,Benopril group and Shenqingyin-Benopril combination group.After 8 weeks＇ treatment,blood glucose,renal function and 24h urine protein were measured,and malondialdehyde（MDA） in renal tissues and urine,superoxide diamutase（SOD） activity and serum transformed growth factor-β1（TGF-β1） were tested;renal pathological changes were observed with light microscope as well.Results The serum BUN,Scre of all medicine groups were significantly improved,which did not significantly differ from that in control group（P〉0.05）.However,it had no obvious improving effect on blood sugar.The content of 24h urine protein in all medicine groups was significantly lower than that in model group（P〈0.05 or P〈0.01）,and Shenqingyin- Benopril combination group had the significantly lower level than the other two medicine groups（P〈0.01）.MDA in renal tissues and urine,SOD activity and serum TGF-β1 in all medicine groups significantly differed from those in the model group（P〈0.05 or P〈0.01）,and Shenqingyin-Benopril combination group had obvious difference with the other two medicine groups（P〈0.01）.Light microscopy showed amelioration of different degree in renal pathological changes,but with no significant differences in all medicine groups.Conclusion Shenqingyin Decoction has a protective effect on the kidney of experimental diabetic rats,and it has some synergetic effect when combined with Benopril.The mechanism may be related to inhibiting oxidative stress,improving the body＇s antioxidant ability and decreasing the expression of inflammatory factor TGF-β1.