益肾胶囊对慢性肾小球肾炎患者sEPCR及CD62P的影响

Effects of Yishen Capsule on sEPCR and CD62P in patients with chronic glomerulonephritis

目的观察益肾胶囊对慢性肾小球肾炎(慢性肾炎,chronic glomerulonephritis,CGN)患者血浆可溶性内皮细胞蛋白C受体(soluble endothelium protein C receptor,sEPCR)、P-选择素(P-selectin,CD62P)及血清免疫球蛋白(Ig)、补体3水平的影响。方法分别采用酶联免疫双抗体夹心法、流式细胞仪及免疫比浊法检测了40例健康查体者和78例患者治疗前后血浆sEPCR、CD62P及血清免疫球蛋白(Ig)、补体3水平的改变。结果治疗前慢性肾炎患者血浆sEPCR、CD62P及血清IgA水平均明显升高(P<0.01),血清IgG、IgM及补体C3水平明显降低(P<0.01),经过3个月的治疗,治疗组患者血浆sEPCR、CD62P及血清IgA水平明显下降(P<0.05或P<0.01),IgG、IgM及补体C3明显升高(P<0.05或P<0.01)。结论益肾胶囊治疗CGN患者的作用机制之一可能与降低血浆sEPCR、CD62P水平及调节血清IgAI、gGI、gM和补体C3水平有关。

Objective To explore the effects of Yishen Capsule on the soluble endothelium protein C receptor（sEPCR） and P-selectin（CD62P） as well as the serum levels of immunoglobulin（Ig） and complement 3（C3） in patients with chronic glomerulonephritis（CGN）.Methods The changes of sEPCR and CD62P levels in plasma as well as Ig and C3 levels in serum were assessed in 40 normal subjects and 78 patients before and after treatment by double-antibody enzyme-linked immunosorbent assay,flow cytometry and immunoturbidimetry,respectively.Results Compared with those in normal group before treatment,the levels of sEPCR and CD62P in plasma as well as IgA in serum increased obviously（P〈0.01）,and the levels of IgG and IgM as well as C3 in serum decreased significantly in CGN patients（P〈0.01）.After three months＇ treatment with Yishen Capsule,the levels of sEPCR and CD62P in plasma and IgA in serum decreased significantly（P〈0.05 or P〈0.01）,and the levels of IgG and IgM in plasma as well as C3 in serum increased obviously（P〈0.05 or P〈0.01）.Conclusion The mechanism of Yishen Capsule＇s effect in treating patients with CGN may be correlated with decreasing sEPCR and CD62P levels in plasma as well as regulating the levels of Ig in serum and C3.