口臭患者舌背菌群分析

Diversity of bacterial population on the tongue dorsa of halitosis patients

目的分析口臭患者舌背菌群多样性变化的规律。方法采集7名口臭患者和4名健康者舌苔标本,使用通用引物扩增标本中所有细菌的16SrDNA片段,测序分析并构建舌背微生物的种系进化树。结果共检测212个克隆,口臭组136个,鉴定出42种微生物(包括9种尚不能确定的微生物);对照组76个克隆,鉴定出23种微生物(包括7种尚不能确定的微生物)。2组标本中检出率最高、检出数目最多的菌种均为链球菌;其中17种已知细菌只在口臭患者舌苔上检出,检出率最高的是叶瘤杆菌(85.7%),殊异韦荣氏菌(71.4%)和Solobacterium moorei(57.1%)。结论口臭组舌背菌群的生物多样性高于对照组。叶瘤杆菌、殊异韦荣氏菌、Solobacterium moorei可能与口臭的产生相关,需进一步研究证实。

Objective To analyze the microbial profiles of the tongue dorsa in halitosis patients. Methods The samples from the dorsal tongue surface were taken from 7 halitosis patients and 4 control subjects without halitosis. 16S rRNA genes of bacteria from the samples were amplified by PCR with universally conserved bacterial primers, cloned into Escherichia coli and sequenced. Phylogenetic trees were constructed by software CLUSTAL X 1.83 and TREEVIEW. Results A total of 212 isolates and phylotypes were identified from the 11 subjects ,42 species （including 9 uncultured species） were found in subjects with halitosis,while 23 species （including 7 uncultured species） were found in control subjects. Streptococcus sp was the most popular species in both groups. The detection rate of TM7 phylum sp was the highest in subjects with halitosis ,followed by Veillonella dispar and Solobacterium moorei. Conclusion The biodiversity of the tongue dorsa of subjects with halitosis was higher than that of subjects without halitosis. TM7 phylum sp, Veillonella dispar,Solobacterium moorei may be related to halitosis.