干旱胁迫下橡胶树叶片差异表达蛋白的鉴定与功能解析

Identification and Functional Analysis of Differentially Expressed Proteins in Hevea brasiliensis Leaves during Drought Stress

干旱是影响橡胶树生长和产胶的重要非生物胁迫。以橡胶树无性系热研7-33-97为试验材料,通过干旱胁迫处理后,提取并纯化样品叶片的总蛋白质,经双向凝胶电泳(2-DE)和胶图分析。结果表明,干旱胁迫与对照样品相比,干旱胁迫10d和15d的橡胶树叶片蛋白中共存在38个差异表达蛋白点。采用MALDI-TOFMS分析和数据库检索,对差异表达的蛋白进行鉴定和功能注释,依照功能分类,这些蛋白主要参与胁迫应激响应、能量代谢、光合作用、信号转导以及细胞定位等生物学过程。

Drought is a serious abiotic stress which affects growth and latex production of rubber tree （Hevea brasiliensis）. Proteomics analysis of rubber tree leaves in response to drought stress has not been reported to date. Total leaf protein was extracted and purified from the rubber tree of Clone Reyan 7-33-97, and then separated by two-dimensional gel electrophoresis（2-DE）. Gel analysis showed that a total of 38 differentially expressed protein spots were found in the 2-DE protein profile of the samples under the drought stress（10 days and 15 days） and the control. MALDI-TOF MS and online searching indicate that these proteins are involved in diverse biological activities such as stress response, energy metabolism, photosynthesis, signal transduction and cellular localization,