荔枝快速碱化因子基因的克隆与表达分析

Cloning and Expression Analysis of LcRALF Gene from Litchi

从已构建的荔枝果皮cDNA文库中挑取克隆测序,获得一条荔枝快速碱化因子(LcRALF)基因(GenBank登录号:EU024484)。该基因的cDNA全长为666bp,含1个381bp的开放读码结构,编码126个氨基酸。生物信息学分析表明,该基因的氨基酸序列与拟南芥、烟草等物种的RALF基因一致性较高,具有RALF基因氨基酸序列的典型特征。RT-PCR分析表明,该基因在荔枝果皮中特异表达,且在新采收的荔枝果皮中表达量最高。随着果皮的衰老该基因表达量逐渐下降。

A novel rapid alkalinization factor gene（LcRALF） of litchi was isolated from cDNA library of litchi pericarp（GenBank accession No.：EU024484）. The LcRALF cDNA was 666 bp in full length with an open reading frame of 381 bp, which encoded a 126 aa polypetide. Amino acid sequence of LcRALF had high identities with RALF genes from Arabidopsis thaliana, Nicotiana tabacum and other plant species and had the same structure characteristics as other RALF genes. RT-PCR analysis showed that LcRALF was expressed specially in litchi pericarp and had the highest expression level in the pericarp of freshly-harvested litchi fruit. With the senescence of pericarp, expression level of LcRALF decreased gradually.