光敏胞质雄性不育小麦NAD激酶和NADP磷酸酶对光周期的反应

RESPONSE OF ACTIVITIES OF NAD KINASE AND NADP PHOSPHATASE IN LEAVES OF PHOTOPERIOD SENSITIVE CYTOPLASMIC MALE STERILE WHEAT TO DIFFERENT PHOTOPERIODS

研究了光敏感胞质雄性不育小麦及其对照可育系，在不同生育期叶片内ＮＡＤ激酶和ＮＡＤＰ磷酸酶的活性对不同光周期的反应．ＬＤ下可育核供体小麦叶片内的ＮＡＤ激酶总活性、ＣａＭ依赖性和ＣａＭ非依赖性ＮＡＤ激酶活性均略高于ＳＤ，但差别不显著；ＮＡＤＰ磷酸酶活性在孕穗、抽穗和籽粒灌浆期均高出ＳＤ一倍以上．光敏胞质雄性不育小麦在ＬＤ和ＳＤ下，叶片中的ＮＡＤ激酶总活性、ＣａＭ依赖性和ＣａＭ非依赖性ＮＡＤ激酶活性以及ＮＡＤＰ磷酸酶活性均有明显差别，而且ＬＤ下ＣａＭ依赖性ＮＡＤ激酶活性逐渐被ＣａＭ非依赖性ＮＡＤ激酶活性所取代．这反应出光敏胞质雄性不育小麦体内的ＮＡＤ激酶和ＮＡＤＰ磷酸酶活性比核供体正常可育系对光周期的反应更敏感，这可能是ＬＤ导致光敏小麦育性转换和花粉败育的原因之一．

The activities of NAD kinase and NADP phosphatase in leaves of photoperiod sensitive cytoplasmic male sterile wheat and its nuclear donor under LD and SD at different growth and development stsges are studied. The total activity of NAD kinase, the activities of CaM dependent and CaM independent NAD kinase in leaves of the nuclear donor Baipi 224 under LD are slightly higher as compared to those under SD. However the activity of NADP phosphatase in leaves of the nuclear donor wheat under LD was two times higher than that under SD at booting, heading and milking stages. The activities of totol NAD kinase, CaM dependent and CaM independent NAD kinase and NADP phosphatase in leaves of the photoperiod sensitive cytoplasmic male sterile wheat under LD were different from those under SD. On the photoperiod sensitive male sterile wheats, with the progressing of development leaf CaM dependent NAD kinase activity dropped gradually while CaM independent NAD kinase activity did the opposite and became the prevailing part of the total NAD kinase activity. It is demonstrated that NAD kinase and NADP phophatase of photoperiod sensitive male sterile wheat are more sensitive to different photoperiod than those of nuclear donor fertile wheat, which is probably one of the factors that results in fertility transformation and pollen abortion of male sterile wheat under LD