嗜碱细菌NTT33碱性β-甘露聚糖酶的纯化与性质研究

THE PURIFICATION AND PROPERTIES OF ALKALINE β MANNANASE PRODUCED BY ALKALOPHILIC BACILLUS SP. NTT33

报道了嗜碱芽孢杆菌ＮＴＴ３３产生的碱性β－甘露聚糖酶经纯化后，在ＰＡＧＥ电泳上呈现一条蛋白带，分子量为３．８９×１０７，等电点为３．０～４．０，酶反应最适ｐＨ为９．０～１０．０，最适作用温度为８０℃，稳定ｐＨ为６．０～９．０，稳定温度为６０℃以下．金属离子Ｃｕ２＋，Ｂａ２＋，Ｍｇ２＋，Ａｌ３＋，Ｃｏ２＋对该酶有一定的激活作用，而Ａｇ＋，Ｈｇ２＋，Ｍｎ２＋对该酶有明显抑制作用，经薄层层析鉴定，该酶水解槐豆胶后产生葡萄糖、甘露糖以及低聚糖．

The purification and properties of alkaline β mannanase produced by alkalophilic Bacillus sp. NTT33 were reported. Only one kind of alkline β mannanase was obtained . The molecular weight and pI value of the β mannanase were 38 900 and 3.0￣4.0 , respectively. The optimal pH and temperiture for β mannanase were 9.0￣10.0 and 80℃. The enzyme showed a high stability below 60℃ and at pH 6.0￣8.0. The activity of the enzyme was enhanced by Cu 2+ ,Ba 2+ ,Mg 2+ , Al 3+ ,Co 2+ and was inhibited by Ag +,Hg 2+ , Mn 2+ . The major components that the enzyme hydrolysed locust bean gum were glucose, mannose and oligosaccharides.