生物发光成像的特点及应用

Bioluminescence imaging characteristics and application

生物发光成像（BLI）是通过荧光素酶基因标记细胞或DNA，在ATP及氧气存在条件下，催化荧光素的氧化反应而发光．从而能够直接监控活体内的细胞活动和基因行为。该文通过比较BLI与MRI，PET、放射成像的异同，以及BLI在肿瘤、干细胞和免疫细胞运输、细胞凋亡等方面的应用，为更好地推广BLI的应用提供依据。

Biolumineseence imaging （BLI） by lueiferase gene marked cells or DNA, in the presence of ATP and oxygen, catalytic oxidation reaction of fluoreseein luminescence. So that it can directly monitor in vivo cell activity and gene behavior. In this paper, by comparing the BLI and MRI. PET, radiography of the similarities and differences, as well as about their cancer, stem cells and immune cells transportation. apoptnsis and other aspects of the application, in order to better provide the basis for promoting the application of BLI.