“百消散”毒理学实验观察

The toxicity experiment observation of Bai-xiao-san(BXS)

目的:对百消散的急性毒性和亚急性毒性进行初步研究。方法:采用最大耐受量测定法及30d连续给药法分别观察其急性毒性和亚急性毒性。结果:急性毒性试验表明,百消散对雌、雄性小鼠急性经口给药LD50分别为10.8 g/kg和9.26 g/kg。亚急性毒性试验结果显示,0.35 g/kg及以上剂量组动物试验一周后活动减少,体重增加缓慢,被毛蓬松无光泽;雌、雄大鼠的体重及增重、食物利用率、脾/体重系数、肾上腺/体重系数、胸腺/体重系数、白细胞总数(WBC)、血红蛋白(HGB)、丙氨酸氨基转移酶(ALT)、总蛋白(TP)、白蛋白(ALB)、胆固醇(TC)等检测参数与对照组比较,均有极显著性差异(P<0.01);甘油三脂(TG)、肌酐(CRE)与对照组比较差异无显著性(P>0.05)。病理组织学检查可见胃粘膜贲门处复层扁平细胞增生明显,表皮角化明显。部分粘膜有炎症反应,表现为炎症细胞浸润,血管扩张。结论:亚急性毒性试验结果显示,百消散可引起动物的体重、肝肾功能、血常规、脏器系数等检测指标的改变,且有一定剂量-反应关系,对此应作进一步研究。

Object ：To observe the acute and sub-acute toxic effects of Bai-xiao-san（BXS）. Method：we adopted maximally tolerated dosage in acute toxicity tests in mice and successive administration 30d for sub-acute toxicity tests in rats. Result ： The acute toxicity showed a dose-dependent increase after oral administration of BXS in mice. The LD50 was observed at 10.8 g/kg and 9.26 g/kg, respectively. sub-acute toxic effects shows that after given dosage above0.35 g/kg a week, the movement of mice reduced, weight increased slowly, skin showed no smooth. The weight and increased weight,food availability,spleen/body-mass index,adrenal/body-mass index,thymus/ body-mass index, WBC, WGB, ALT,TP, ALB ,TC had significant difference compared to control group（ P 〈 0.01 ） ;TG, CRE showed no differece to control group（ P 〉 0. 05 ）. Accrementition of flat cell was seen through histopathologic examination at gastric mucosa cardia, scarfskin cuticularizate obviously, part of membrana mucosa had Inflammatory reaction. Conclusion：The study shows that at common dosage BXS would not cause drug interaction. However, at high dosages it exhibited marked damage to all the major organs of the body.