摘要
目的将p73β基因转染胰腺癌BxPC-3细胞,观察其对胰腺癌细胞生物活性的影响。方法将pcDNA3.1-N0和pcDNA3.1-p73β基因转染胰腺癌BxPC-3细胞,用MTT法进行细胞凋亡检测。结果该扩增片段经限制性核酸内切酶酶切鉴定,p73β基因相对表达量明显高于BxPC-3细胞和空载质粒转染细胞BxPC-3-pcDNA3.1-N0两对照组(P<0.05)。与两对照组相比,重组质粒转染细胞pcDNA3.1-p73β实验组细胞增殖明显减弱(P<0.05)。结论包含人类p73基因TAp73β转录本蛋白编码区的cDNA片段被成功扩增,其末端带有限制性核酸内切酶XbaⅠ、KpnⅠ识别序列,且对胰腺癌细胞有抑制作用。
Objective To assess the inhibition of p73β on human pancreatic carcinoma Bxpc-3 cell in vitro.Methods pcDNA3.1-N0 and pcDNA3.1-p73β were transfected into BxPC-3 cell line by LipofectAMINE 2000.Cell apoptosis was detected by MTT.Results The recombinational plasmid pcDNA3.1-p73β was identified by restriction endonuclease.The expression of p73β in BxPC-3-pcDNA3.1-p73β cells was significantly higher than those in control group of BxPC-3 and BxPC-3-pcDNA3.1-N0 cells（P〈0.05）.The proliferative ability of BxPC-3-pcDNA3.1-p73β was much more inhibited than in controls（P〈0.05）.Conclusion p73 gene TAp73β CDS fragment which encodes TAp73β protein is successfully amplified,in particular it is designed XbaⅠ、KpnⅠ recognition enzyme site,and p73β inhibit the proliferation of pancreatic carcinomas cells.
出处
《江西医学院学报》
CAS
2009年第11期21-22,26,F0003,共4页
Acta Academiae Medicinae Jiangxi
