杂化硅胶基质C_(16)整体柱制备及电色谱性能

Preparation and Evaluation of Organic-inorganic Hybrid C_(16) Monolithic Silica Column for Capillary Electrochromatography

采用有机-无机杂化sol-gel技术,两步法制备了杂化硅胶基质C16毛细管电色谱整体柱,考察了理论塔板高度与流动相线速度之间的关系及流动相中乙腈含量、离子强度对电渗流的影响,以烷基苯同系物探讨了反相电色谱分离作用机理,并在反相色谱条件下分离了4种苯胺类碱性物质。实验结果表明,由于采用了杂化基质,有效地减小了苯胺类碱性物质的峰拖尾。

A hybrid matrix C16 silica monolith was prepared by two steps of organic-inorganic hybrid solgel technique. The relationship between plot of plate height （HEPT） and the linear velocity was searched. Then the effects of ACN content in mobile phase and buffer concentration on electroosmotic mobility were performed,and the reversed phase chromatographic behavior of hybrid matrix C16 silica monolith was evaluated with alkylbenzenes. Finally, four anilines were successfully separated. The results showed that the prepared monolithic silica column can avoid peak tailoring of anilines effectively because of the hybrid silica-based.