基于差异蛋白质组学解析一贯煎对大鼠肝硬化形成的影响

Effects of Yiguanjian Decoction on liver cirrhosis formation:a differential proteomics study in rats

目的:基于差异蛋白质组学探讨一贯煎干预肝硬化的部分效应机制。方法:48只Wistar雄性大鼠随机分为正常组(n=12)及造模组(n=36),采用50%四氯化碳(carbonte tra-chloride,CCl4)-橄榄油溶液1mL/kg腹腔注射(2次/周,共9周)制备大鼠肝硬化模型。于开始造模后3周和6周分别随机处死6只大鼠,作药物干预前的动态观察,其余24只模型大鼠于开始造模后第7周首日随机分为模型组(n=12)及一贯煎干预组(n=12)。大鼠于第9周末全部处死取材。双向凝胶电泳(two-dimensional gel electrophoresis,2-DE)分离肝组织总蛋白,基质辅助激光解吸电离飞行时间串联质谱及数据库查询鉴定差异表达蛋白质;差异蛋白质铜/锌超氧化物歧化酶(Cu/Zn superoxide dismutase,Cu/Zn SOD)和DJ-1表达采用蛋白印迹法及免疫组织化学方法进行验证。结果:获得分辨率高、重复性好的肝组织2-DE图谱,经质谱鉴定的50个差异蛋白质中,与氧化应激相关的5个蛋白质分别是Cu/ZnSOD、DJ-1、谷胱甘肽合成酶、谷胱甘肽S-转移酶Yb-1亚基、醛酮还原酶7,A2。与正常组大鼠比较,模型组肝组织Cu/ZnSOD、DJ-1、谷胱甘肽S-转移酶及醛酮还原酶7,A2的表达均显著降低,其中Cu/ZnSOD、醛酮还原酶7,A2在造模3周、6周及9周后呈梯级下降,而DJ-1、谷胱甘肽S-转移酶于造模3周、6周及9周后均呈低水平表达;谷胱甘肽合成酶表达显著升高。治疗结束后与模型组比较,一贯煎组Cu/ZnSOD、DJ-1、谷胱甘肽S-转移酶及醛酮还原酶7,A2表达均明显增高,谷胱甘肽合成酶表达显著降低。蛋白印迹法及免疫组织化学验证了Cu/ZnSOD、DJ-1在蛋白表达水平的差异,与蛋白质组学结果基本一致。结论:抗氧化应激功能降低是CCl4致大鼠肝硬化的重要病理环节,提高抗氧化应激功能相关蛋白的表达可能是一贯煎治疗CCl4所致大鼠肝硬化的主要效应机制。

Objective： To investigate the effects of Yiguanjian Decoction, a compound traditional Chinese herbal medicine, on rats with cirrhosis based on the method of differential proteomics. Methods： Wistar male rats （n=48） were randomly divided into normal control group （n=12） and model-making group （n=36）. Rat cirrhosis model was established by intraperitoneal injection of 50% carbon tetrachloride （CCl4） plus olive oil solution （1 mL/kg, twice weekly for 9 weeks）. After 3- and 6-week injection, 6 rats each time were sacrificed for dynamic observation before medicine intervention, and the 24 remained rats were randomly divided into untreated group （n=12） and Yiguanjian Decoction group （n=12） at the first day of the 7th week. All animals were sacrificed by the end of the 9th week, and total protein of liver tissue was isolated by two-dimensional gel electrophoresis （2-DE）; some differentially expressed protein spots were analyzed and identified by matrix-assisted laser desorption/ionization two-stage time-of-flight mass spectrometry （MALDI-TOF/TOF-MS） and database querying. Protein expressions of Cu/Zn superoxide dismutase （Cu/Zn SOD） and DJ-1 were validated by Western blot and immunohistochemical methods. Results： 2-DE maps with high resolution and good repeatability were obtained. In all 50 protein spots identified by MALDI-TOF/TOF-MS and database querying, there were 5 protein spots related to oxidative stress named Cu/Zn SOD, DJ-1, glutathione synthetase, glutathione S-transferase Yb-1 subunit and aldo-keto reductase family 7, A2 respectively. Compared with the normal control group, expressions of Cu/Zn SOD, DJ-1, glutathione S-transferase Yb-1 subunit and aldo-keto reductase family 7, A2 in the untreated group were decreased significantly. Expressions of Cu/Zn SOD and aldo-keto reductase family 7, A2 were decreased time-dependently. Compared with the untreated group in 9th week, protein expressions of Cu/Zn SOD, DJ-1, glutathione S-transferase Yb-1 subunit and aldo-keto reductase family 7, A2 in the Yiguanjian Decoction groups were increased significantly while expression of glutathione synthetase was decreased notably. Western blot and immunohistochemical results of Cu/Zn SOD and DJ-1 expressions coincided with proteomics results. Conclusion： Anti-oxidative depression is a key pathological change of cirrhosis induced by CCl4 in rats, and increasing expression of proteins related to anti-oxidative stress may be a major mechanism of Yiguanjian Decoction in treating cirrhosis induced by CCl4 effectively.