HPLC快速测定发酵液中L–色氨酸

Quick Determination of L-Tryptophan in Fermented Broth by HPLC

建立一种测定发酵液中L–色氨酸的高效液相色谱(HPLC)方法.以Agilent C18(5μm,150.mm×4.6.mm)为分离柱,V(0.03%的KH2PO4溶液)﹕V(甲醇)=90﹕10为流动相,流量为1 mL/min,在278.nm处检测L–色氨酸.该方法简便、快速、准确,在0.000～1.000.g/L范围内线性关系良好(R2=0.999 9),平均回收率为98.93%,精密度(RSD)为0.247%,可有效应用于发酵液中L–色氨酸含量的测定.

A method to determine L-tryptophan in fermented broth was developed by high performance liquid chromatography （HPLC）. An Agilent C18 （5 μm, 150 mm×4.6 mm） was used with the mobile phase of 0.03% KH2PO4 water solution and methanol （the ratio of volume was 90 to 10）. The flow rate was at 1.0 mL/min and the detective wavelength was 278 nm. The method was rapid, accurate and simple with a good linearity （R2=0.999 9）in the range of 0.000 to 1.000 g/L for L-tryptophan. The average recovery was 98.93% with the relative standard deviation of 0.247%. The method was applicable for L-tryptophan determination in the fermented broth.