基于母离子排除的串联质谱数据采集方法的研究

Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions

生物样本中的蛋白质复杂程度很高,尚无一种分析方法能够全面分析复杂生物样本中的所有蛋白质。实验证明:重复的质谱分析能增加蛋白质的鉴定数量,但鉴定到的蛋白质冗余度很高,高丰度肽段被反复检出。为了降低鉴定冗余度,提高鉴定效率,对线性离子阱-傅立叶变换离子回旋共振质谱仪的串联质谱采集方法进行了研究,建立了基于母离子排除的串联质谱采集方法。此方法能极大降低二级质谱采集的冗余度,提高蛋白质的鉴定效率。

Due to the complexity of proteome samples,comprehensive analysis to characterize all proteins was still not possible with present methodologies.It has been shown that replicate runs could increase the number of identified proteins.However,the redundancy of protein identifications was high.High-abundant peptides tended to be analyzed repeatedly in different runs.To reduce the redundancy and improve the efficiency of identification,we studied the MS/MS acquisition method of linear ion trap Fourier transform ion cyclotron resonance-mass spectrometry（LTQ-FT） and an acquisition strategy based on exclusion of precursor ions was developed.It proved that the strategy could extremely reduce the redundancy of MS/MS acquisition and improve the efficiency of protein identifications.