响应曲面法优化大肠杆菌XD-12发酵培养基的研究

Study on Optimization of Fermentation Medium of Escherichia coli XD-12 for Aminotransferase Production by Response Surface Methodology

[目的]采用响应面法优化大肠杆菌XD-12发酵培养基,以提高转氨酶的转化得率。[方法]在单因素试验基础上,采用响应曲面法对菌种的发酵培养基进行了优化研究。[结果]最佳的培养基组成为:葡萄糖10.0 g/L,玉米浆28.3 ml/L,蛋白胨9.3g/L,MgSO_4 0.5g/L,NaCl 0.5g/L,pH值7.2,在此条件下,酶转化得率的预测最优值为93.8%,实际平均值为93.0%。[结论]模型能够较好地预测实际发酵情况,用于大肠杆菌发酵产酶培养基的优化是可行的。

[Objective]The research aimed to optimize the fermentation medium of E.coli XD-12 for amiontransferase production by response surface methodology,to enhance the yield of L-phenylalanine.[Method]Based on the single factor test,fermentation medium of Escherichia coli XD-12 for aminotransferase production was optimized by response surface methodology.[Result]The results showed that the composition of the optimal medium was glucose 10.0 g/L,corn steep liquor 28.3 ml/L,peptone 9.3 g/L,MgSO_4 0.5 g/L,NaCl 0.5 g/L,pH value 7.2.Under this condition,the maximum yield of L-phenylalanine was 93.8%as the predictive value and 93.0%as the practical value.[Conclusion] The model can better predict the real fermentation situation,and it is feasible to be used to optimize the fermentation medium of E.coli.