扯根菜黄化苗转绿过程中4种生理指标的动态变化

Dynamic Change of 4 Physiological Indexes in Yellowing Seedlings of Penthorum chinense during Green-turning Process

[目的]探讨光照对扯根菜生长发育的影响。[方法]采用组织培养法获取扯根菜组培苗和黄化苗。每隔6 d分别取样,共6次。研究扯根菜黄化苗转绿过程中茎段内脯氨酸、叶绿素、可溶性蛋白含量以及POD活性的动态变化。可溶性蛋白含量测定采用考马斯亮蓝G-250染色法;POD活性测定采用愈创木酚比色法;游离脯氨酸含量测定采用3%磺基水杨酸法;叶绿素测定采用乙醇提取法。[结果]黄化苗的叶绿素和可溶性蛋白含量以及POD活性均低于正常苗,而脯氨酸含量高于正常苗;黄化苗见光后的30 d内,叶绿素含量和POD活性一直处于上升趋势,并最终与正常苗相近;可溶性蛋白与脯氨酸的含量总体呈现先升后降的趋势,最终也接近正常苗;表明黄化苗的光形态建成基本完成。

[Objective]The study aimed to explore the effect of illumination on the growth and development of Penthorum chinense.[Method] The tissue culture plantlets and yellowing seedlings of P.chinense were obtained by using tissue culture method.Their samples were taken in every interval of 6 d resp.with 6 times in total to study the dynamic change of proline,chlorophyll,soluble protein contents and POD activity in the stem segments of yellowing seedlings of P.chinense during g-turning process.The coomassie brilliant blue dyeing method was used to determine the soluble protein content,the guaiacol colorimetric method was used to measure the POD activity,the 3%sulphosalicylic acid was used to measure the free proline and the ethanol extraction method was used to determine the chlorophyll content.[Result]The chlorophyll and soluble protein contents and the POD activity in the yellowing seedlings were all lower than that in the normal seedlings,but the proline content in the yellowing seedlings was higher than that in the normal seedlings.During the 30 d after the yellowing seedlings was in the exposure of the light,the chlorophyll content and POD activity in the yellowing seedlings were in the rising trend at all the time and was close to the mat in the normal seedlings finally.The soluble protein and proline contents in the yellowing seedlings showed a trend of first rising and then dropping in generally and were close to that in the normal seedlings finally,indicating that the photomorphism of the yellowing seedlings was finished basically.