麻疯油转酯化产物的高效液相色谱分析

HPLC Analysis of Jatropha Curcas Oil Product by Transesterification

建立了高效液相色谱法同时测定麻疯油转酯化产物中4种主要脂肪酸甲酯的分析方法。样品经膜处理后用丙酮溶解,采用Hypersil ODS（C18）色谱柱进行分离。以乙腈为流动相进行等度洗脱,内标法定量,同时对色谱条件进行优化。结果表明,在优化的色谱条件下4种脂肪酸甲酯在10 min内得到良好的分离,标准曲线的线性相关系数均达到0.999以上,平均回收率为96%~98%,重现性相对标准偏差为7.2%~10.2%,重复性相对标准偏差为0.31%~2.02%。与气相色谱法相比,该方法具有较高的灵敏度,可用于麻疯油转酯化产物中脂肪酸甲酯含量的测定,为麻疯油转酯化反应制备生物柴油的定性定量分析提供了参考依据。

A high performance liquid chromatographic（HPLC） method was established for the determination of four common fatty acid methyl esters by the transesterification of jatropha curcas oil.After membrane treatment,the samples were dissolved in acetone and separated on a Hypersil ODS（C18） chromatographic column（4.6 mm×250 mm,5 μm）.The separation and analysis of four fatty acid methyl esters was performed on the column by isocratic eluting using acetonitrile as mobile phase with flow rate of 1 mL/min at 40 ℃ and detection wavelength of 210 nm.The internal standard method was used to quantitatively detected and the chromatographic conditions were optimized.Under the optimized conditions,four fatty acid methyl esters were well separated in 10 min.The correlation coefficients of four fatty acid methyl esters were more than 0.999.The average recoveries ranged from 96% to 98% with RSDs of reproducibility of 7.2%-10.2% and RSDs of repeatability of 0.31%-2.02%.The method could be used for the determination of fatty acid methyl esters in the transesterification product of jatropha curcas oil and provided reliable reference for preparation of biodiesel by transesterification.