摘要
在室温条件下的酸性介质中，大豆水溶性蛋白质能与偶氮洋红G（azocarmine G）迅速结合，生成紫红色复合物，该复合物的最大吸收波长为580nm，比偶氮洋红G红移了50nm，其吸光强度与大豆水溶性蛋白质的质量浓度在3～34μg·mL^-1范围内存在较好的选择性和较宽的线性关系。依此建立了测定大豆水溶性蛋白质的新方法，同时优化了大豆水溶性蛋白质的提取过程。该方法具有快速、稳定的特点，与凯氏法所得测定结果基本一致。
A purple-red complex could be formed rapidly between azocarmine G and water-soluble soybean protein in acidic medium. The absorbance of this complex had a maximum absorption wavelength at 580 nm and exhibited a bathochromic shift of 50 nm compared with the maximum absorption wavelength of azocarmine G. Its absorbance with the concentration of soluble proteins at least in 3 - 34 μg · mL^-1 was linearly. A method on rapid measurement of water-soluble soybean protein was established based on above-mentioned. Simultaneously we optimized the extraction procedure of water- soluble soybean protein. The results obtained by this method was rapid, stable. And the results were same with that obtained by Kjeldahl＇s method.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第1期124-126,共3页
Soybean Science
