利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮

Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins

以染料木素为指标,采用紫外分光光度法测定异黄酮的含量,并利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮。结果表明:该树脂分离淡豆豉异黄酮的工艺参数为:上样液浓度0.225 g.mL-1,pH值4.5,最大上样量150 mL,以4 BV.h-1的流速吸附,以8倍柱床体积的70%乙醇作为洗脱剂,洗脱流速4 BV.h-1,树脂可重复使用2次。经初步吸附分离,异黄酮得率达40%以上。

Use ultraviolet spectrophotometry to detect the content of soybean isoflavone, with the content of genistein as the index, to investigate the ADS-7 macroporous adsorptive resins for separation and purification of soybean isoflavone in the Semen Sojea Praeparatum. The optimized technological parameters of the ADS-7 macroporous adsorptive resins were as following：loading sample density 0.225 g · mL^-1 ,pH 4.5 ,the maximum sample volume 150 mL,4 BV · h^-1 flow rate to adsorption and the volume of 70% （v/v） ethanol as 8 BV with desorption-flow 4 BV· h^- 1. With these conditions, more than 40% of soybean isoflavone could be obtained from primary adsorption and purification, and the ADS- 7 macroporous adsorptive resins can be used twice.