摘要
该文报道了研制的一套细胞灌注装置,并运用该细胞灌注装置与核磁共振连用的方法在线监测药物作用下肿瘤细胞的能量代谢过程.结果表明核磁共振细胞灌注方法可有效观察到细胞能量代谢的变化,可作为追踪筛选药物的活性成分及探索药物治疗肿瘤细胞的作用机理的一种有力手段.
A cell perfusion system for online NMR studies is reported. The system was used to measure ^31p NMR signals of ATP in living breast cancer cells （MCF-7）, with or without perfusion with cantharidin solution. The results showed that online NMR on perfused cell culture is an effective way to identify active components from extracts of traditional Chinese medicine. The method can also be used to study the interactions between drugs and cancer cells.
出处
《波谱学杂志》
CAS
CSCD
北大核心
2010年第1期89-94,共6页
Chinese Journal of Magnetic Resonance
基金
国家自然科学基金资助项目(20765001)
贵州省科技基金资助项目[黔科合J字(2006)2012]
